This product is a lyophilized micro-core (primers and probes not included) prepared from the liquid lyo-ready formulation of the Hifair™ Lyo-Ready Multiplex One Step RT-qPCR Kit (UDG plus)(Cat#16645ES). It enables multiplex quantitative PCR reactions using RNA as a template. By combining reverse transcription and quantitative PCR in a single reaction tube, this kit simplifies experimental procedures and minimizes the risk of contamination.

Utilizing advanced lyophilization technology, this product transforms liquid reagents into stable lyophilized micro-cores. This design significantly facilitates storage and transportation while enhancing the overall stability of the reagents.

Features

• Broad Compatibility: Suitable for direct detection or freeze-dried for long-term storage
• Strong stability: after freeze-drying, the stability is still good at 50℃ for 25 days
• Support fast program: the fastest 40 minutes to get the result
• Significant anti-pollution effect: UDG/dUTP anti-pollution system, easy to remove contamination.

Application

Virus detection; pathogen detection; gene expression analysis

Specifications

Components

Storage

This product should be stored at -25~-15℃ for one year. 

Figures

1. Freeze-dried products form 

Figure 1. Freeze-dried product form(Eight-row freeze-dried micropheres)

2. Consistent performance

Figure 2. Consistent performance between Liquid reagent and lyophilized Microspheres

3. Stability test—Appearance stability of freeze-dried microspheres

Figure 3. Freeze-dried microspheres accelerate stability((fast procedure)

After being freeze-dried into freeze-dried microspheres using Yeasen's RNA lyophilizable reagent (Catt#16936ES),  placed under different conditions (accelerated at 50°C for 33 days), and 10⁴, and 10³ copies/mL of HPIV pseudovirus templates were amplified simultaneously. The results showed that there was no significant change in the Ct value, fluorescence value, spherical shape, etc. of the freeze-dried microspheres before and after thermal acceleration, and the freeze-dried product had good stability.

4. Mutiplex PCR Amplification of pathogens

Figure 4. Multiplex amplification of pathogens

(HPIVs, Template concentration E6/5/4/3/2/1) 

Documents:

Safety Data Sheet

16936_MSDS_HB20260408.PDF

Manuals

16936_Manual_HB20260407.pdf