説明
FuniCutTM series endonucleases are fast restriction enzymes developed through genetic engineering that can precisely cut DNA within 5–15 minutes. These enzymes are suitable for rapid digestion of plasmid DNA, PCR products, genomic DNA, and more. All FuniCutTM fast restriction enzymes share the same digestion buffer, which simplifies reaction setup. Additionally, they have a high enzyme activity margin, making them capable of easily handling excess substrate or challenging templates.
Specifications
|
Cat. No. |
15010ES78 |
|
Size |
600 T |
|
Recognition Site |
5'-G↓AATTC-3' 3'-CTTAA↑G-5' |
|
Recommended Reaction Conditions |
1× FuniCutTM Buffer; incubate at 37°C |
|
Enzyme activity |
20 U/μL |
|
Inactivation Conditions |
Incubate at 80°C for 20 minutes. |
|
Cohesive-end ligase |
FunII |
Components
|
Components No. |
Name |
15010ES78 |
|
15010-A |
FuniCutTM EcoRI |
600 μL |
|
15010-B |
10×FuniCutTM Buffer |
3×1 mL |
|
15010-C |
10×FuniCutTM Color Buffer* |
3×1 mL |
【Note】: 10× FuniCutTM Color Buffer includes red and yellow tracking dyes, allowing direct loading of reaction products onto agarose gels without additional loading buffer. In 1% agarose gel electrophoresis, the red dye co-migrates with ~2500 bp double-stranded DNA fragments, while the yellow dye migrates similarly to ~10 bp double-stranded DNA fragments.
Shipping and Storage
This product should be stored at -25℃~-15°C for up to 2 years.
Documents:
Safety Data Sheet
Manuals
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