説明
The FuniCut™ series of restriction enzymes are engineered via recombinant DNA technology to enable precise DNA digestion within 5–15 minutes. These fast-acting restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, genomic DNA, and other substrates. All enzymes in the FuniCut™ series share a universal digestion buffer, simplifying reaction setup. Additionally, they exhibit high activity redundancy, enabling efficient digestion even with excess substrate or challenging templates.
Specifications
|
Enzyme |
Basl |
|
Recognition Site |
5'-GGTCTC(N)₁↓-3' 3'-CCAGAG(N)₅↑-5' |
|
Recommended Reaction Conditions |
1× FuniCut™ Buffer; incubate at 37°C. |
|
Enzyme Activity |
20 U/μL |
|
Inactivation Conditions |
Incubate at 80°C for 20 minutes. |
|
Isolytic enzymes |
PalAI and SgsI |
|
Methylation modification affects |
Dam, Dcm, EcoKI, and EcoBI: No effect; CpG: Complete sequence overlap, cleavage blocked. |
Components
|
Components No. |
Name |
15005ES50 |
|
15005-A |
FuniCut™ BsaI |
50 μL |
|
15005-B |
10×FuniCut™ Buffer |
1 mL |
|
15005-C |
10×FuniCut™ Color Buffer |
1 mL |
[Note]:*10xFuniCut™ Color Buffer includes red and yellow tracking dyes, allowing direct use of the product for gel electrophoresis. The red dye migrates similarly to a 2500 bp double-stranded DNA fragment in 1% agarose gel; the yellow dye migrates similarly to a 10 bp double-stranded DNA fragment in 1% agarose gel.
Shipping and Storage
This product should be stored at -25℃~-15℃ for up to 2 years.
Documents:
Safety Data Sheet
Manuals
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