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Hieff Unicon™ Robust Hotstart Taq DNA Polymerase, Glycerol-free (20 U/μL) is an antibody-mediated hot-start DNA polymerase with high amplification sensitivity and specificity. The blocking antibody is fully inactivated within 30 seconds at the initial denaturation temperature, releasing DNA polymerase activity. This hot-start mechanism helps suppress amplification caused by nonspecific primer annealing. The glycerol-free formulation is designed specifically for preparing lyophilized reagents.
Features
Lyophilization-Ready: Glycerol-free liquid formulation optimized for lyophilized reagent production.
High Inhibitor Tolerance: Compatible with complex samples (swabs, blood); lyophilized beads support full-volume reconstitution with nucleic acid extracts.
High Performance: Antibody-mediated hot-start ensures sensitive and specific amplification.
Robust Stability: Retains >90% activity after 7 days at 37°C or 20 freeze-thaw cycles.
Components
|
Name |
14331ES76 |
14331ES80 |
14331ES92 |
14331ES93 |
|
Hotstart Taq DNA Polymerase, Glycerol-free (20 U/μL) |
50 μL |
500 μL |
1 mL |
5 mL |
Application Examples
1. Strong Stability
Two batches of 14331 were evaluated for accelerated thermal stability and repeated freeze–thaw stability, with material stored at -20℃ as the control. After 7 days at 37℃ or 20 freeze–thaw cycles, both batches retained more than 90% of the activity measured under standard storage conditions.
Figure 1. Accelerated thermal stability and freeze–thaw stability of two batches of 14331.
2. African Swine Fever Virus Detection with Lyophilized Beads
|
Item |
Content |
|
Reagents |
14331 was combined with qPCR buffer, ASFV primers/probes, and lyoprotectants to prepare 25 μL lyophilized qPCR reagents |
|
Samples |
Eight farm whole-blood samples were extracted by magnetic beads; 400 μL input, 70 μL elution |
|
Testing |
Lyophilized reactions were reconstituted with 5 μL or 25 μL nucleic acid extract, topped up to 25 μL with water |
|
Results |
25 μL reconstitution gave Ct values ~2–4 cycles earlier than 5 μL, indicating tolerance to higher extract input and improved sensitivity |
Table 1. Detection of eight field samples using 14331-based lyophilized qPCR reagents.
3. HPV Detection with Lyophilized Beads
|
Item |
Content |
|
Reagents |
14331 was combined with qPCR buffer, HPV primers/probes, and lyoprotectants to prepare 15 μL lyophilized qPCR reagents |
|
Samples |
Ninety-eight HPV clinical samples were extracted by magnetic beads; 200 μL input, 100 μL elution |
|
Testing |
Each lyophilized reaction was fully reconstituted with 15 μL nucleic acid extract |
|
Results |
Both 14331-based and comparator reagents detected all 98 samples; 14331 gave lower Ct in 43, equal in 24, and higher in 31 |
Table 2. Detection of 98 HPV clinical samples using 14331-based lyophilized qPCR reagents.
Storage
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