説明
Hieff Clone™ Zero TOPO-TA/Blunt Cloning Kit is developed based on the principle of a next-generation topoisomerase that enables rapid and efficient ligation of DNA fragments. Coupled with a highly efficient blunt-ending factor, this kit supports fast cloning of both blunt-ended and sticky-ended PCR products.
Features
- Fast: Ligation completes in just 5 minutes.
- Highly Efficient: Virtually no self-ligation; near 100% positive clone rate—no blue-white screening needed.
- Broad Insert Range: Supports fragments from 150 bp to 5 kb.
- Simple Workflow: Just add your purified insert—no extra steps required.
- Versatile Compatibility: Enhanced with blunt-end binding factor for seamless TA cloning and blunt-end cloning.
Components
|
Components No. |
Name |
10906ES08 (5T) |
10906ES20 (20 T) |
10906ES50 (50 T) |
|
10906-A |
2× Hieff Clone™ Universal TOPO Cloning Premix |
25 μL |
100 μL |
250 μL |
|
10906-B |
1 kb control insert (40 ng/μL) |
10 μL |
10 μL |
10 μL |
|
10906-C |
M13 Forward Primer(10 μM) |
50 μL |
200 μL |
500 μL |
|
10906-D |
M13 Reverse Primer(10 μM) |
50 μL |
200 μL |
500 μL |
Storage
This product should be stored at room temperature for 1 year.
Application
Molecular Cloning; Topo Cloning; Rapid Cloning
Figures
1. Versatile Compatibility: TA cloning and blunt-end cloning.
Figure 1. High cloning efficiency of the Hieff Clone™ Universal Zero TOPO TA/Blunt Cloning Kit.
With 10 ng of a 1 kb insert (either blunt- or sticky-ended), the kit yields abundant colonies and achieves 100% positive clone rate.
2. Stong Stability
(A).Accelerated Stability
(B).Freeze-Thaw Stability
Figure 2. Stability Assessment
(A).Accelerated Stability: The Hieff Clone® Universal Zero TOPO TA/Blunt Cloning Kit remains stable after 7 days at 37°C.
(B).Freeze-Thaw Stability: The kit maintains performance after 50 freeze-thaw cycles.
Documents:
Safety Data Sheet
Manuals
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