شرح
IL-2 R beta is a member of the cytokine receptor superfamily . Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain.
Specifications
|
Cat.No. |
96268ES25 / 96268ES60 / 96268ES76 |
|
Size |
25 μg / 100 μg / 500 μg |
Components
|
Name |
96268ES25 |
96268ES60 |
96268ES76 |
|
Recombinant Cynomolgus IL-2 R beta/CD122 Protein,His Tag,Ultra Low Endotoxin |
25 μg |
100 μg |
500 μg |
Performance parameters
|
Synonyms |
CD122;IL-15 R beta;IL-2 R beta;IL-2R subunit beta;IL2RB;P70-75;p75;RP5-1170K4.6;IL-15 R β;IL-2 R β;IL-2R subunit β |
|
Species |
Cynomolgus |
|
Expression Sequence |
Ala27-Asp239 |
|
Accession |
Q38J85 |
|
Tag |
C-His |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 25.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and SEC-HPLC |
|
Endotoxin |
Less than 0.001 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological activity |
SPR Data: Cynomolgus IL-2 R beta, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.17 μM as determined in SPR assay . |
|
Reconstitution Method |
Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles. |
Storage
Store at -25 to -15°C for up to 1 year from the date of receipt; once reconstituted, store at -85 to -65°C where it remains stable for 3 months, avoiding repeated freeze-thaw cycles.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves,for your safety.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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