Alternative Names

PDCD1, PD1, CD279, SLEB2, PD-1

Species

Canine

Expression Sequence

Leu25-Val170

Accession

NP_001301026.1

Tag

C-hFc

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 43.00 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Canine PD-1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1, mFc Tag with the EC50 of 30.7ng/ml determined by ELISA .

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.