Alternative Names

PLAU, Urokinase, ATF, UPA, URK, u-PA, BDPLT5, QPD

Species

Mouse

Expression Sequence

Gly21-Phe433

Accession

P06869

Tag

C-His-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 49.00 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 30-35 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【SPR Data】Human uPAR, hFc Tag captured on CM5 Chip via Protein A can bind Mouse PLAU, His-Avi Tag with an affinity constant of 10.24 μM as determined in SPR assay .

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.