Alternative Names

SCFR, PBT, CD117, ckit, c-kit, KIT

Species

Cynomolgus,Rhesus macaque

Expression Sequence

Gln26-Pro520

Accession

XP_005555332.1 / AFE78183.1

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 56.69 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Cynomolgus/Rhesus macaque CD117, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA . 【ELISA Data】 Immobilized Human SCF, No Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD117, His Tag with the EC50 of 4.76μg/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.