Alternative Names

CD269, TNFRSF17, BCMA, BCM, TNFRSF13A

Species

Cynomolgus,Rhesus macaque

Expression Sequence

Met1-Ala53

Accession

A0A2K5UD97 / F7FFA0

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 7.10 kDa. Due to glycosylation, the protein migrates to 13-23 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Cynomolgus BCMA, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer), His Tag with the EC50 of 7.4ng/ml determined by ELISA. 【ELISA Data】 Immobilized Cynomolgus BCMA, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-BCMA Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.