Alternative Names

HLA G antigen, sHLA-G, b2 microglobulin, HLA G, HLAG, HLA-G, MHC-G, sHLA-G

Species

Cynomolgus

Expression Sequence

Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide

Accession

E0WKX9(HLA-G)&Q8SPW0(B2M)&RIIPRHLQL

Tag

C-His-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【SPR Data】Cynomolgus LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Cynomolgus HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag with an affinity constant of 0.13 μM as determined in SPR assay .

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.