Alternative Names

αVβ3, Integrin alpha V beta 3, beta3, alphaV, ITGAV&ITGB3,Integrin alpha V β 3, β3

Species

Cynomolgus

Expression Sequence

Phe31-Pro993(ITGAV) acidic tail & Gly27-Asp718(ITGB3) basic tail

Accession

A0A2K5WCD3(ITGAV)&A0A2K5WYN9(ITGB3)

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 112.31 kDa (ITGAV) & 81.27 kDa (ITGB3). Due to glycosylation, the protein migrates 95-140 to kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】Immobilized Cynomolgus ITGAV&ITGB3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-alpha V beta 3 Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.