Alternative Names

CLMF, CLMF2, IL-12A, IL-12B, IL12, IL12 p70, IMD28, IMD29, NFSK, NKSF1, NKSF2, P35, IL-12 subunit p35, IL12A, interleukin 12

Species

Cynomolgus

Expression Sequence

Ile23-Ser328 (IL-12B) & Arg57-Ser253 (IL-12A)

Accession

G7P6S2(IL-12B)&XP_005546300.2(IL-12A)

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 34.76 kDa (IL-12B) and 23.64 kDa (IL-12A). Due to glycosylation, the protein migrates to 45-48 kDa (IL-12B) and 38-44 kDa (IL-12A) based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

ELISA Data: Immobilized Cynomolgus IL-12, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.

Reconstitution Method

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.