Alternative Names

Alkaline Phosphatase, ALP-1, Alkaline phosphatase, placental-like, GCAP, PLAP-like, ALPG, ALPPL, ALPPL2

Species

Human

Expression Sequence

Ile20-Asp503

Accession

P10696

Tag

C-His-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 55.48 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE,> 90% as determined by HPLC

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Anti-Alkaline Phosphatase Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Alkaline Phosphatase (Germ type) , His Tag with the EC50 of 23.4ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.