Alternative Names

CD3e, CD3E, T3E, FLJ18683, TCRE, CD3-epsilon, IMD18

Species

Human

Expression Sequence

Asp23-Asp126

Accession

P07766

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 12.6 kDa. Due to glycosylation, the protein migrates to 14-18 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Anti-CD3 Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human CD3E, His Tag with the EC50 of 1.9ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.