Alternative Names

NCAM1,NCAM,NCAM-1,N-CAM-1

Species

Human

Expression Sequence

Leu20-Gly718

Accession

P13591-2

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 78.48 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】Immobilized Human NCAM-1, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 5.1 ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.