Alternative Names

MACAM1,MADCAM1,MAdCAM-1

Species

Human

Expression Sequence

Gln19-Gln317

Accession

Q13477-1

Tag

C-hFc

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 58.17 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Human MADCAM1, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-MADCAM1 Antibody, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.