Alternative Names

beta3,ITGAV&ITGB3Integrin alpha V beta 3,αVβ3

Species

Human

Expression Sequence

Phe31-Val992(ITGAV) acidic tail&GLy27-Asp718(ITGB3) basic tail

Accession

P06756-1(ITGAV)&P05106-1(ITGB3)

Tag

C-His-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 114 kDa (ITGAV)&81.3 kDa (ITGB3). Due to glycosylation,the protein migrates to 85-140 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Human ITGAV&ITGB3, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA.

Reconstitution Method

Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.