Property Name

Property Details

Alternative Names

STK-1, FLK2, CD135

Species

Human

Expression Region

Asn27–Asn541

Accession

P36888-1

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 59.22 kDa. Due to glycosylation, the protein migrates to 80–110 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Bioactivity

[ELISA Data] Immobilized Human FLT3, His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with an EC₅₀ of 12.3 ng/mL.

Reconstitution

Centrifuge the tube before opening. Reconstitute the lyophilized protein in distilled water to a concentration greater than 100 μg/mL. It is recommended to aliquot after reconstitution to avoid repeated freeze-thaw cycles.