Property Name

Property Details

Alternative Names

KGFR, Keratinocyte growth factor receptor, FGFR-2, Fibroblast growth factor receptor 2

Species

Human

Expression Region

Pro253–Glu378

Accession

P21802-3

Tag

C-Avi

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 15.49 kDa. Due to glycosylation, the protein migrates to 35–50 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Bioactivity

[ELISA Data] Immobilized Human FGFR2 beta (IIIb) Domain, Avi Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose-response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag, with an EC₅₀ of 1.6 ng/mL determined by ELISA.

Reconstitution

Centrifuge the tube before opening. Reconstitute the lyophilized protein in distilled water to a concentration greater than 100 μg/mL. It is recommended to aliquot after reconstitution to avoid repeated freeze-thaw cycles.