Property Name

Property Details

Alternative Names

notch ligand DLL4, Delta-like 4, MGC126344, DLL4

Species

Human

Expression Region

Ser27–Pro524

Accession

Q9NR61

Tag

No Tag

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 54.28 kDa. Due to glycosylation, the protein migrates to 55–70 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, 200 mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Bioactivity

【ELISA Data】 Immobilized Human DLL4, No Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 8.7 ng/mL determined by ELISA.

Reconstitution

Centrifuge the vial before opening. Reconstitute lyophilized protein using distilled water at a concentration above 100 μg/mL. It is recommended to aliquot the reconstituted protein for single-use to avoid repeated freeze-thaw cycles.