Product Alias

CT83, HLA class I, MHC class I, HLA-A, HLA-A*0101, MHC, MHC HLA-A alpha

Species

Human

Expression sequence

Gly25-Thr305(HLA-A*01:01),Ile21-Met119(B2M) and NTDNNLAVY peptide

Accession

Q5SUL5(HLA-A*01:01)&P61769(B2M)&NTDNNLAVY

Label

C-His-Avi

Expression system

HEK293

Molecular weight

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

preparation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.