Nature name

Nature Details

Product Alias

HLAE, sHLA-E, HLAE, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, MHC, QA1

Species

Human

Expression sequence

Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M) and VMAPRTLVL peptide

Accession

P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL

Label

C-His-Avi

Expression system

HEK293

Molecular weight

The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

purity

> 95% as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per μg by the LAL method.

preparation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.。