Nature name

Nature Details

Product Alias

MHC, KRAS, K-Ras 2, KRAS2, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KRAS1, KRAS2, NS, NS3, RASK2, GTPase Kras, KI-RAS, RALD

Species

Human

Expression sequence

Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M) and VVVGAVGVGK peptide

Accession

NP_002107.3 (HLA-A*03:01)&P61769 (B2M)&VVGAVGVGK

Label

C-His-Avi

Expression system

HEK293

Molecular weight

Approximately 50.2 kDa.Due to glycosylation, the protein migrates to55-60 kDa based on Tris-Bis PAGE result.

purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

preparation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【SPR Data】Human KRAS G12V (HLA-A*03:01) Complex, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-KRAS G12V (HLA-A*03:01) Antibody with an affinity constant of 0.205μM as determined in SPR assay (Biacore T200).

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.。