شرح
PDGF is a disulfide-linked dimer consisting of two 12.0-13.5 kDa polypeptide chains, named PDGF-A and PDGF-B. PDGF is stored in platelet α-granules and released upon platelet activation. PDGF is involved in many biological processes, including proliferation, chemotaxis, embryonic neuronal development, and respiratory tubule epithelial cell development.
Use sterile deionized water to reconstitute the freeze-dried protein. It is recommended to divide the protein into aliquots when using it for the first time to avoid repeated freezing and thawing.
Features
- Recombinant human PDGF-AA derived from CHO cells.
- Provided in the form of lyophilized powder.
- High activity, high purity.
- Low endotoxin.
- No labeling.
Applications
The three naturally occurring PDGF-AA, PDGF-BB, and PDGF-AB are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells.
Specifications
|
Synonyms |
Fibroblast Growth Factor-4, HST-1, Transforming protein KS3, HBGF-4 |
|
Species |
Human |
|
Source |
CHO Cells |
|
Sequence |
Ser87-Thr211 |
|
Accession |
P04085 |
|
Tag |
None |
|
Molecular Weight |
Predicted molecular mass：14.3kDa(monomer) |
|
Endotoxin |
≤10 EU/mg by the LAL method |
|
Purity |
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
|
Activity |
The activity is determined by the dose-dependent proliferation of NIH3T3 cells is typically between ≤5.0 ng/mL. |
|
Formulation |
Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4 |
|
Appearance |
Lyophilized powder |
Components
|
Components No. |
Name |
91612ES10 |
91612ES10 |
91612ES80 |
|
91612 |
Recombinant Human PDGF-AA Protein(CHO-expressed) |
10 μg |
50 μg |
1 mg |
Shipping and Storage
Store at -25~-15℃, valid for 1 year from the date of receipt. After reconstitution, store at 2~8°C, valid for 7-10 days. After reconstitution, store at -85~-65℃, valid for 6 months.
Documents:
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FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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