شرح
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced by by mast cells, activated T cells and bone marrow stromal cells. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively.It is a potent cytokine produced by T cells and to a lesser extent by tumor-associated natural killer cells, basophils, and mast cells. IL-4 is a differentiation factor for transforming growth factor-beta secreting Th3. And IL-4 activates two distinct signalling pathways through tyrosine phosphorylation of Stat6, a signal transducer and activator of transcription, and of a 170K protein called 4PS.
Components
|
Components No. |
Name |
90144ES08 |
90144ES20 |
90144ES50 |
90144ES60 |
90144ES76 |
|
90144 |
Recombinant Mouse IL-4 Protein |
5 μg |
20 μg |
50 μg |
100 μg |
500 μg |
Specifications
|
Synonyms |
Interleukin-4, IL-4, BSF-1, BCDF, IL4E12, Pitrakinra |
|
Accession |
|
|
GeneID |
|
|
Source |
E.coli-derived mouse IL-4 protein, His21-Ser140 |
|
Molecular Weight |
Approximately 13.5 kDa |
|
AA Sequence |
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
> 97% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
The ED50 as determined by the dose-dependant prolifiration of Murine HT-2 cells is less then 2 ng/mL, corresponding to a Specific Activity of > 5 × 105 IU/mg. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 0.1-1.0 mg/mL. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Storage
This product should be stored at -25~-15℃ for 1 year. Once reconstituted under sterile conditions, it can be stored at 2 ~ 8°C for 7 days, or at -85 to -65°C for 3 months.
Reconstitution Instructions
Centrifuge the vial briefly before opening to pellet the contents at the bottom. Reconstitute with sterile deionized water to a final concentration of 0.1–1.0 mg/mL. Further dilution can be performed as needed for downstream applications. For long-term storage, it is recommended to add a carrier protein (e.g., 0.1% BSA) to the dilution buffer. Aliquot the solution into single-use portions and store at -80°C to avoid repeated freeze-thaw cycles.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves，for your safety.
Documents:
Safety Data Sheet
Manuals:
Payment & Security
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.