شرح
Interleukin-1β (IL-1β) is a pro-inflammatory cytokine produced by a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. IL-1α and IL-1β bind to the same receptor and exhibit similar biological properties, although they are not entirely identical. These cytokines display a broad spectrum of activities, including stimulating thymocyte proliferation by inducing IL-2 release, promoting B cell maturation and proliferation, exhibiting mitogenic FGF-like activity, and inducing the release of prostaglandins and collagenases from synovial cells.
This recombinant human IL-1β is supplied as a lyophilized powder. It features high activity, high purity, low endotoxin levels, and is tag-free.
Components
|
Components No. |
Name |
90140ES05 |
90140ES10 |
90140ES50 |
90140ES60 |
90140ES76 |
|
90140 |
Recombinant Mouse IL-1β Protein |
2 μg |
10 μg |
50 μg |
100 μg |
500 μg |
Specifications
|
Synonyms |
Interleukin-1 beta, IL-1β, IL-1, IL1B, IL-1b, IL1-BETA, IL-1F2, IL1F2IL-1 beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta. |
|
Source |
E.coli-derived Mouse IL-1β protein, Vla118-Ser269. |
|
Accession |
|
|
Molecular Weight |
Approximately 17.5 kDa. |
|
AA Sequence |
VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS |
|
Endotoxin |
< 1.0 EU/μg by the LAL method. |
|
Purity |
> 96% as determined by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/mL, corresponding to a specific activity of > 5.0 × 108 IU/mg. Fully biologically active when compared to standard. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5% trehalose, 0.02% Tween-20. |
|
Tag |
No |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Storage
This product should be stored at -25~-15℃ for 1 year. Once reconstituted under sterile conditions, it can be stored at 2 ~ 8°C for 7 days, or at -85 to -65°C for 3 months.
Reconstitution Instructions
Centrifuge the vial briefly before opening to pellet the contents at the bottom. Reconstitute with sterile deionized water to a final concentration of 0.1–1.0 mg/mL. Further dilution can be performed as needed for downstream applications. For long-term storage, it is recommended to add a carrier protein (e.g., 0.1% BSA) to the dilution buffer. Aliquot the solution into single-use portions and store at -80°C to avoid repeated freeze-thaw cycles.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves，for your safety.
Documents:
Safety Data Sheet
Manuals:
Payment & Security
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
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