MycAway™ Plus-Color One-Step Mycoplasma Detection Kit _ 40612ES
MycAway™ Plus-Color One-Step Mycoplasma Detection Kit _ 40612ES
MycAway™ Plus-Color One-Step Mycoplasma Detection Kit _ 40612ES
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MycAway™ Plus-Color One-Step Mycoplasma Detection Kit _ 40612ESMycAway™ Plus-Color One-Step Mycoplasma Detection Kit _ 40612ESMycAway™ Plus-Color One-Step Mycoplasma Detection Kit _ 40612ES
MycAway™ Plus-Color One-Step Mycoplasma Detection Kit _ 40612ES
The MycAway™ Plus-Color One-Step Mycoplasma Detection Kit is a rapid detection product for mycoplasma contamination in cell culture which was developed based on the unique isothermal amplification technology of YEASEN. Compared with the previous mycoplasma detection products, this kit is optimized and has greatly reduced the false positive rate, improved the accuracy of the test, and enhanced the identification of negative and positive results. At the end of amplification, the sample can be left at room temperature for a period of time without the change from negative to positive, and...

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MycAway™ Plus-Color One-Step Mycoplasma Detection Kit _ 40612ES

ذخیره کنید $120.00
YeasenSKU: 40612ES25
Size: 25 T
قیمت:
$115.00 $235.00
انبار:
در انبار

The MycAway™ Plus-Color One-Step Mycoplasma Detection Kit is a rapid detection product for mycoplasma contamination in cell culture which was developed based on the unique isothermal amplification technology of YEASEN. Compared with the previous mycoplasma detection products, this kit is optimized and has greatly reduced the false positive rate, improved the accuracy of the test, and enhanced the identification of negative and positive results. At the end of amplification, the sample can be left at room temperature for a period of time without the change from negative to positive, and thus will not affect the judgment of the results.

The principle is that if the cell culture is contaminated with mycoplasma, the conserved sequences of mycoplasma DNA will be amplified heavily and rapidly, causing the color of the reaction solution to change from blue-purple to sky-blue, and the result is visible to the naked eye, without electrophoresis.

MycAway™ Plus-Color One-Step Mycoplasma Detection Kit can detect multiple mycoplasma species, including eight that are common in cell culture. The traditional nested PCR method for mycoplasma detection is susceptible to influence by the inhibitors in cell culture supernatant and showed a false-negative result. Also if needed to perform the electrophoresis, cap opening may increase the contamination and lead to a false-positive result. The one-step mycoplasma detection kit has none of these disadvantages, also its detection sensitivity and accuracy are much higher than those of the PCR method.

Yeasen offers comprehensive solutions for Mycoplasma contamination. Explore our related products: Concept of Mycoplasma and the Impact of Contamination

Feature

The one-step operation results in less than 60 minutes

Use a water bath or PCR instrument to test

22 mycoplasma species were accurately detected by the kit, including 8 common species

Results just identified by the visual inspection

Application

Mycoplasma Detection

Components

Components No. Name 40612ES25 （25 T) 40612ES60 (100 T)
40612-A MycAway™ -Color Lamp 425 μL 4 × 425 μL
40612-B MycAway™-Color Lamp Primer 50 μL 4 × 50 μL
40612-C Positive Control 10 μL 4 × 10 μL
40612-D Mineral oil 500 μL 4 × 500 μL

Figures

  • The detection procedure

Figure 1. The detection procedure by MycAway™ Plus–Color One-Step Mycoplasma Detection Kit from YEASEN

    Storage

    All the components are shipped with dry ice and can be stored at -15℃ ~ -25℃ for 18 months. Please keep away from the light.

    FAQ

    This kit contains UDG enzyme to remove the aerosol contamination of PCR product containing dU, which greatly inhibits false positive reactions and improves the accuracy of the test. Moreover, the added UDG enzyme functions at room temperature and is temperature-sensitive and prone to inactivation, without affecting the experimental results. If the cell culture is contaminated by mycoplasma, the conserved sequences of mycoplasma DNA will be amplified in large quantities and rapidly, causing the reaction solution to change from blue-purple to sky-blue. The result can be easily identified by the naked eye and no electrophoresis is required.
    This reagent kit is much simpler to use. Just take 1 μL of the suspected mycoplasma-contaminated cell culture supernatant and add it to the reaction system. First, place the sample at 25-37 ℃ for 5 minutes, then incubate it at a constant temperature of 63 ℃ in a water bath or PCR instrument for 60 minutes. Observe that the reaction solution changes from blue-purple to sky-blue, and the result can be clearly seen without the need for electrophoresis.
    The mycoplasma kit is unstable and prone to degradation in ethanol, DMSO organic solvents, and in environments with strong acids and strong bases. As a result, the color changes to a sky blue. This kit can only be used for cell detection and should not be used in organic solvents or in environments with strong acids and strong bases for detection.
    40612 Principle: Mg²⁺+HNB = Blue color. As PCR increases, pyrophosphate accumulates continuously. Pyrophosphate combines with Mg²⁺, while HNB remains free, resulting in a sky blue color. This reagent contains sodium dihydrogen phosphate, which may be the reason for the immediate chemical reaction between this ion and Mg²⁺ causing the reaction to occur and change color. EDTA can chelate calcium and magnesium ions, thereby accelerating the color change reaction.
    10 to the power of 5 copies per ul.
    No, it's a linearized segment.

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    Inquiry

    FAQ

    The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.

    Certain applications may require additional third-party intellectual property rights.

    Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.