Anti-Flag affinity purification gel (secretory protein or Membrane Protein) is prepared by covalent coupling of high-quality mouse IgG2b monoclonal antibody (clone number: 3F5) and 4% highly cross-linked agarose gel. This product has a high loading capacity of Flag-tagged fusion protein (at least 1.1 mg protein/mL gel) and less non-specific binding of impurities. It can be used for purification and immunoprecipitation (IP) of fusion proteins (secretory protein or membrane protein) with Flag tags.

Anti-Flag affinity purification gel (secretory protein or Membrane Protein) can bind to Met-modified N-terminal Flag fusion protein (Met-FLAG–Protein), N-terminal Flag fusion protein (FLAG–Protein), and C-terminal Flag fusion protein (Protein-FLAG).

Features

High specificity: High-quality mouse IgG2b monoclonal antibody is used, which can specifically adsorb the Flag protein sequence.

High loading capacity: Loading capacity ≥ 1.1 mg protein/mL gel. However, the capacity of Anti-FLAG M2 Affinity gel is >0.6 mg/mL.

High purity: Less non-specific binding of impurities, resulting in high-purity Flag-tagged protein.

Wide range of uses: Can be used for protein purification or IP.

Multiple types of binding proteins: Can bind to Met-modified N-terminal Flag fusion protein (Met-Flag–Protein), N-terminal Flag fusion protein (Flag–Protein), C-terminal Flag fusion protein (Protein-Flag)

Specifications

Application

Purification of secreted or membrane proteins, immunoprecipitation（IP

Figures

Improved Yield and Purity

Figure 1. Anti-DYKDDDDK (Flag) Affinity Gel has higher yield and purity of secretory or membrane proteins compared to other suppliers.

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