شرح
The Hieff™ Endo-free Plasmid Midi Kit enables rapid purification of 100–500 µg of high-purity, high-copy plasmid DNA from 30–70 mL of E. coli LB (Luria-Bertani) culture. The spin column utilizes a proprietary silica-based matrix developed by our company, combined with a unique lysis buffer formulation, to maximize recovery of high-quality plasmid DNA. A specialized endotoxin-removal process ensures extremely low endotoxin levels (<0.1 EU/µg DNA), resulting in excellent performance in cell transfection. The purified plasmid is also directly suitable for a wide range of downstream molecular biology applications, including restriction digestion, transformation, PCR, in vitro transcription, sequencing, and more.
Features
High Stability: Specially designed silica membranes in spin columns ensure minimal variation between columns and excellent reproducibility.
High Yield: Quickly and easily extract 100–500 µg of high-copy plasmid DNA from 30–70 mL E. coli LB culture.
Low Endotoxin: Unique formulation reduces endotoxin to <0.1 EU/µg DNA, ideal for efficient cell transfection.
Components
|
Components No. |
Name |
19023ES02 |
19023ES20 |
|
|
Part I |
19023-A |
RNase A (10 mg/mL) |
50 µL |
500 µL |
|
Part II |
19023-B |
Endotoxin Removal Solution ER (ER Solution E2) |
1.8 mL |
18 mL |
|
19023-C |
RS Buffer* (RS Buffer E2*) |
5 mL |
50 mL |
|
|
Part III |
19023-D |
AC Buffer (AC Buffer E2) |
1 mL |
10 mL |
|
19023-E |
Hieff™ DNA Column E2 |
2 columns |
20 columns |
|
|
19023-F |
15 mL Collection Tube E2 |
2 tubes |
20 tubes |
|
|
19023-G |
Lysis Buffer LB (LB Buffer E2) |
5 mL |
50 mL |
|
|
19023-H |
Binding Buffer BD (BD Buffer E2) |
5 mL |
50 mL |
|
|
19023-I |
Protein Removal Buffer PL* (PL Buffer E2*) |
4 mL |
40 mL |
|
|
19023-J |
Wash Buffer W* |
2.5 mL |
25 mL |
|
|
19023-K |
Elution Buffer |
2 mL |
20 mL |
|
Storage
Part I: Store at –25°C to –15°C
Part II: Store at 2–8°C
Part III: Store at room temperature
Valid life: 2 years
Documents:
Safety Data Sheet
Manuals
Payment & Security
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.