Hieff™ Endo-free Plasmid Mini Kit is designed for rapid extraction of 20–50 µg of high-purity plasmid DNA from 1–10 mL of E. coli LB culture. This kit features high efficiency, speed, and convenience. The spin column utilizes a proprietary silica-based matrix material developed by our company. Combined with our unique lysis buffer formulation, it enables maximum recovery of high-purity DNA. The extracted DNA has high purity and extremely low endotoxin residue (<0.1 EU/µg DNA), resulting in excellent performance in cell transfection. The DNA can be directly used in various molecular biology applications such as restriction digestion, transformation, PCR, in vitro transcription, and sequencing.
Feature
Stable performance: Specially designed silica membranes ensure consistent DNA binding and reliable results.
Fast and easy: Extract 20–50 µg high‑copy plasmid DNA from 1.5–5 mL E. coli LB culture in about 30 minutes.
Pure DNA: Removes endotoxins to below 0.1 EU/µg DNA for excellent cell transfection.Specifications
Components
|
Category |
Components No. |
Name |
19021ES08 |
19021ES50 |
19021ES70 |
|
Part I |
19021-A |
RNase A (10 mg/mL) |
15 µL |
125 µL |
500 µL |
|
19021-B |
Endotoxin Removal Solution ER (ER Solution E2) |
500 µL |
5 mL |
20 mL |
|
|
Part II |
19021-C |
Buffer RS* (RS Buffer E2*) |
1.5 mL |
12.5 mL |
50 mL |
|
Part III |
19021-D |
Buffer AC (AC Buffer E2) |
500 µL |
5 mL |
20 mL |
|
19021-E |
DNA Binding Column E2 (Hieff™ DNA Column E2) |
5 pcs |
50 pcs |
200 pcs |
|
|
19021-F |
2 mL Collection Tube (2 mL Collection Tube E2) |
5 pcs |
50 pcs |
200 pcs |
|
|
19021-G |
Lysis Buffer LB (LB Buffer E2) |
1.5 mL |
12.5 mL |
50 mL |
|
|
19021-H |
Binding Buffer BD (BD Buffer E2) |
1.5 mL |
12.5 mL |
50 mL |
|
|
19021-I |
Protein Lysis Buffer PL* (PL Buffer E2*) |
1.6 mL |
16 mL |
63 mL |
|
|
19021-J |
Wash Buffer W* (Wash Buffer*) |
1.3 mL |
13 mL |
50 mL |
|
|
19021-K |
Elution Buffer |
1 mL |
10 mL |
20 mL |
Application
plasmid DNA Extraction; Endo-free Plasmid Extraction; Plasmid preparation for transfection.
Figures
1. Superior plasmid quality with ultra‑low endotoxin
Figure 1. Plasmid DNA extracted using Yeasen 19021, Supplier O*, and Supplier T* kits was transfected into HEK293 cells with pEGFP‑C1. Bright‑field and green‑fluorescence images were taken at 72 h post‑transfection, showing transfection efficiency in the order Yeasen 19021> Supplier T* > Supplier O*.
Storage
FAQ
Bacterial strain issue: Plasmids were lost during the preservation of the bacterial strain. Before culturing, perform streaking to activate the bacteria, which will lead to a stable production output.
The bacterial cells were not fully lysed: Resuspend them thoroughly in the buffer to avoid clumping;
Reagent preparation error: The solution with precipitate formation needs to be heated; The volume of added ethanol is incorrect.
Insufficient elution: Preheat the elution solution and perform a second elution.
Citations
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Inquiry
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.