شرح
The Magnetic Bead-Based Sample Pretreatment Kit (Bottle Format, Fast Version) is designed for the pretreatment of biological samples, including sample types such as antibody therapeutics and viral vectors used in gene and cell therapy. This kit utilizes unique magnetic beads and a carefully optimized buffer system to enable rapid and efficient isolation and purification of trace DNA from complex sample matrices. A manual nucleic acid extraction can be completed within 30 minutes. Additionally, the kit is fully compatible with automated nucleic acid extraction instruments (16-, 48-, and 96-channel), enabling efficient high-throughput extraction.
This sample pretreatment kit can also be used in conjunction with various host cell residual DNA detection kits and risk gene detection kits from our company, including the following kits: Vero Residual DNA Detection Kit (Cat#41307ES), E. coli Residual DNA Detection Kit (Cat#41308ES),HEK293 Residual DNA Detection Kit (3G) (Cat#41331ES),CHO Residual DNA Detection Kit (3G) (Cat#41332ES),SV40 LTA & E1A Residual DNA Detection Kit (Cat#41310ES).
Features
- Fast and Easy Operation – Complete DNA extraction in just 30 minutes.
- High Recovery Rate – Host residual DNA recovery ranging from 70% to 130%.
- High Purity – Extracted DNA is free of proteins, salts, and detergents.
- Flexible Extraction Options – Compatible with both manual workflows and automated nucleic acid extraction instruments, suitable for different sample volumes.
- Excellent Compatibility – Extracted DNA can be used directly with detection kits from other manufacturers.
Specifications
|
Cat NO. |
18469ES25 / 18469ES60 |
|
Size |
25 T / 100 T |
Components
|
Category |
Component No. |
Component Name |
18469ES25 |
18469ES60 |
|
Part I |
18469-A |
Proteinase K (20 mg/mL) |
0.5 mL/tube × 1 tube |
1 mL/tube × 2 tubes |
|
18469-B |
Glycogen |
225 μL/tube × 1 tube |
900 μL/tube × 1 tube |
|
|
18469-C |
Poly A potassium salt |
150 μL/tube × 1 tube |
600 μL/tube × 1 tube |
|
|
18469-D |
Precipitation enhancer |
100 μL/tube × 1 tube |
400 μL/tube × 1 tube |
|
|
Part II |
18469-E |
Magnetic Bead Suspension |
0.5 mL/tube × 1 tube |
1 mL/tube × 2 tubes |
|
18469-F |
Lysis and Binding Buffer |
15 mL/bottle × 1 bottle |
60 mL/bottle × 1 bottle |
|
|
18469-G |
Wash Buffer A* |
6 mL/bottle × 1 bottle (add 9 mL ethanol) |
24 mL/bottle × 1 bottle (add 36 mL ethanol) |
|
|
18469-H |
Wash Buffer B* |
3 mL/bottle × 1 bottle (add 12 mL ethanol) |
12 mL/bottle × 1 bottle (add 48 mL ethanol) |
|
|
18469-I |
Elution Buffer |
2.5 mL/bottle × 1 bottle |
10 mL/bottle × 1 bottle |
Storage
1. Part I components are shipped on dry ice and should be stored at -25°C ~ -15°C. Valid for 1 year.
2. Part II components are shipped at room temperature. Component 18469-E (Magnetic Bead Suspension) is recommended to be stored at 2–8°C; other components can be stored at room temperature. Valid for 1 year.
Application
- Preparation of residual DNA from cell lines, viruses, and bacteria.
- Quality control of biopharmaceutical products.
- Downstream use in residual DNA detection assays.
Figures
1. High Recovery Rate: Spike recovery rates within 80%–120%
Figure 1. Spike recovery of E. coli host cell DNA at three concentrations using the Magnetic Sample Preparation Kit with the E. coli Residual DNA Detection Kit (2G, Cat#41308). Recovery rates ranged 80%–120%.
2. Broad Compatibility: Compatible with manual and automated extraction
Figure 2. Comparison of host cell DNA extraction efficiency using four different methods with the S. cerevisiae Host Cell DNA Residue Detection Kit (Cat# 41324). Spiked samples at three concentration levels were processed, and no significant differences were observed among the extraction methods.
3. Excellent Performance with High Recovery
Table 1. CT Values and Recovery Rates for Different Samples and Extraction Methods
Documents:
Safety Data Sheet
Manuals
18469_Manual_Ver.EN20250901.pdf
Related Blog
High-sensitivity, fg-grade, residual DNA detection kits for quality control of biological products
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