eSpCas9 mRNA encodes an engineered Streptococcus pyogenes Cas9 (eSpCas9) protein with enhanced expression specificity. When delivered into cells or in vivo for CRISPR-based gene and cell therapies, it expresses the S. pyogenes Cas9 nuclease with more than 10-fold reduction in off-target effects, while maintaining robust on-target editing efficiency.

This mRNA features a Cap1 structure to enhance translation efficiency and protein expression levels, and includes a 100-nucleotide poly(A) tail that mimics mature eukaryotic mRNA, significantly improving its stability.

Specifications

Components

Storage

Store at –80°C. Valid for 2 years

Notes

1. This product is for research use only.

2. For your safety and health, please wear a lab coat and disposable gloves when handling.

3. Upon receipt, immediately store the sample at –80°C.

4. During first use, wear disposable gloves, thaw the sample on ice, and use RNase-free reagents and consumables to avoid contamination.

5. After initial thawing, briefly centrifuge the sample gently and aliquot to avoid repeated freeze-thaw cycles.

Documents:

Safety Data Sheet

17105_MSDS_HB26120_EN.PDF

Manuals

17105_Manual_Ver.EN20260120.pdf