RT-LAMP Probe Assay Kit ( Hotstart Bst Plus, HSS) _ 16903ES
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RT-LAMP Probe Assay Kit ( Hotstart Bst Plus, HSS) _ 16903ES
RT-LAMP Probe Assay Kit ( Hotstart Bst Plus, HSS) _ 16903ES
The  RT-LAMP Probe Assay  Kit is a  reagent designed for  LAMP amplification  using  DNA and  RNA templates.  It includes the next-generation Bst Plus HSS DNA Polymerase, heat-resistant Hifair™  III Reverse Transcriptase, MRI, and an optimized buffering system, allowing for rapid and efficient LAMP/RT-LAMP probe-based detection. The Bst Plus  HSS  DNA  Polymerase employs an aptamer-based hot start mechanism that suppresses enzymatic activity at ambient temperatures, enhancing specificity and sensitivity while enabling stable reaction assembly at room temperature. Features Hotstart Bst DNA Pol High Sensitivity Components   Components No.   Name  ...

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RT-LAMP Probe Assay Kit ( Hotstart Bst Plus, HSS) _ 16903ES

YeasenSKU: 16903ES60
Size: 100 T
قیمت:
$325.00
انبار:
در انبار

The RT-LAMP Probe Assay Kit is a reagent designed for LAMP amplification using DNA and RNA templates. It includes the next-generation Bst Plus HSS DNA Polymerase, heat-resistant Hifair™ III Reverse Transcriptase, MRI, and an optimized buffering system, allowing for rapid and efficient LAMP/RT-LAMP probe-based detection. The Bst Plus HSS DNA Polymerase employs an aptamer-based hot start mechanism that suppresses enzymatic activity at ambient temperatures, enhancing specificity and sensitivity while enabling stable reaction assembly at room temperature.

Features

Hotstart Bst DNA Pol

High Sensitivity

Components

Components No.

Name

16903ES60
(100 T)

16903-A

4×RT-LAMP Buffer (HSS)

600 µL

16903-B

RT-LAMP Enzyme mix (HSS)

100 µL

Specifications

Detection method

RT-LAMP Fluorescent probe method

Enzyme mix

Bst Plus HSS DNA Polymerase; Hifair™ III Reverse Transcriptase

Type of sample

RNA

Application

Isothermal amplification reaction

Operating Instructions

Components

Volume (μL)

Final Concentration

4×RT-LAMP Buffer(HSS)

6

1 ×

RT-LAMP Enzyme mix(HSS)

1

-

Primer mix*

2.5

-

Template

1-10

-

ddH2O

up to 25

-

Notes: Recommended primer set at 16 μM FIP/BIP, 2 μM F3/B3, and 4 μM LoopF/LoopB; the probe can be added at the optimal amount confirmed by the design scheme, and the ratio of other optimal primer sets can be tested.

Reaction conditions

Incubate at a constant temperature of 63-65°C for 30-60 minutes, followed by deactivation at 85°C for 5 minutes.

Notes

For your safety and health, please wear lab coats and disposable gloves for operation.

Storage

The product should be stored at -15 ℃ ~ -25℃ for 1 year. Please avoid light.

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