FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
Features
Rapid digestion: DNA was digested rapidly by enzyme for 5-15 min
Universal buffer: one tube reaction, simplifying the process
Stringent quality control
Applications
Molecular cloning
Southern blotting
SNP
Southern blotting
Genotyping
Specifications
| Enzyme | DpnI |
| Cutting Site | 5'-GAm6↓TC-3' 3'-CT↑Am6G-5' |
| Optimal Reaction Temperature | 37℃ |
| Sensitive to Heat Inactivation | YES |
| Type IIs RE | NO |
| Methylation Sensitivity | Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not EcoKI Methylation-Sensitive, EcoBI Methylation-Sensitive |
| Star Activity | 3 h incubation did not show star activity, and delayed enzyme digestion might show star activity |
| Isoschizomers | NO |
Components
| Components No. | Name | 15052ES50 (50 T) |
| 15052-A | FuniCut™ DpnI | 50 μL |
| 15052-B | 10× FuniCut™ Buffer | 1 mL |
| 15052-C | 10× FuniCut™ Color Buffer | 1 mL |
Storage
FAQ
Citations
Documents
| Manual |
|---|
| https://seas.ysbuy.com/prod/doc/mainDoc/15052_Manual_HB230113_EN.pdf |
Applications
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Inquiry
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.