Recombinant Ten-Eleven Translocase 2.0 (TET 2.0) is an engineered Fe²⁺ and α-ketoglutarate (α-KG)-dependent dioxygenase based on TET enzyme modification and recombinant expression. Through a series of oxidation reactions, it can convert 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC), then into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). With subsequent treatment using a conversion reagent or enzymatic method, 5caC is further converted into uracil (U). Through PCR, uracil (U) is converted into thymine (T), enabling DNA methylation site identification with single-base resolution and high accuracy.

Product Information

Component

Storage

The product should be stored at -25°C to -15°C for 1 year.

Instructions for use

The recommended enzyme input is 30 μg per reaction*. The reaction temperature of this enzyme is 37°C. A complete reaction generally requires 2 h, and a termination step is required.

*: The enzyme input amount can be appropriately adjusted according to experimental requirements.

Notes

1. This enzyme should be handled on ice after thawing.

2. This enzyme is suitable for methylation enzyme-mediated conversion, with a DNA template input of 10-100 ng.

3. This product is for research use only.

4. For your safety and health, please operate with lab coats and disposable gloves.

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