شرح
Endonuclease IV (also known as Nfo) is an endonuclease whose main function is to recognize apurinic/apyrimidinic (AP) sites in DNA strands. It cleaves the phosphodiester bond at the 5′ side of the AP site, generating a 3′-hydroxyl group and a 5′-deoxyribose phosphate terminus (dRP). In addition, this enzyme also has 3′-phosphodiesterase activity and can release glyceraldehyde-3-phosphate, intact deoxyribose 5′-phosphate, and inorganic phosphate from the 3′ end of DNA.
Product Information
|
Item |
Specification |
|
Catalog No. |
14543ES80 / 14543ES90 |
|
Size |
1,000 U / 5,000 U |
|
Source |
E. coli |
|
Molecular Weight |
32.5 kDa |
|
Unit Definition |
One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34 bp oligonucleotide duplex containing one AP site in a 10 μL reaction system at 37°C for 1 h. AP sites are generated by treating a 34 bp oligonucleotide duplex containing one uracil base with 1 U of uracil-DNA glycosylase (UDG, Cat. No. 14455ES) at 37°C for 2 min. |
|
Storage Buffer |
10 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.15% Triton™X-100, 200 μg/mL BSA, 50% Glycerol, pH 7.4 at 25°C. |
|
Concentration |
10 U/μL |
|
Purity |
≥90% (SDS-PAGE) |
Component Information
|
Component No. |
Product Name |
14543ES80 |
14543ES90 |
|
14543-A |
Endonuclease IV (10 U/μL) |
100 μL |
500 μL |
|
14543-B |
10× Endonuclease IV Buffer |
0.8 mL |
1.25 mL |
Applications
- Hydrolyzing AP sites in FFPE sample repair to generate nicks, providing key starting sites for DNA repair.
- Single-cell gel electrophoresis (comet assay).
- Alkaline elution and alkaline unwinding.
Feature
- No non-specific DNase residue.
- Host (E. coli) gDNA residue < 0.05 copies/10 U.
Figure
Case Study 1: No Non-specific DNase Residue
In a 20 μL reaction system, 100 U of Endonuclease IV from different batches and 0.5 μg of λDNA-HindIII digest were added and incubated at 37°C for 16 h. The DNA band pattern did not change in agarose gel electrophoresis, indicating that Yeasen Endonuclease IV contains no non-specific DNase residue.
Figure 1: Non-specific DNase residue.
Case Study 2: Host (E. coli) gDNA Residue < 0.05 copies/10 U
Host (E. coli) genomic DNA residue was tested for different batches of Yeasen Endonuclease IV. The results show that the host gDNA residue of Yeasen Endonuclease IV is < 0.05 copies/10 U.
Figure 2: Host gDNA residue.
Storage
Store at -25°C to -15°C. The shelf life is 1 year.
Published Literature
(1) PAM-engineered abasic-site probe-assisted RPA–CRISPR platform for rapid and sensitive detection of Klebsiella pneumoniae and carbapenem-resistance genes
Journal: SENSORS AND ACTUATORS B-CHEMICAL | DOI: 10.1016/j.snb.2026.140175 | IF: 8.3
Documents:
Safety Data Sheet
Manuals:
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