ssDNA Assay Kit is a simple, sensitive, and accurate single-strand DNA (ssDNA) fluorescence quantitative detection kit with a good linear relationship between 1-200 ng. This kit contains a fluorescence detection reagent, buffer solution, and related ssDNA standards. Before use, dilute the buffer solution of fluorescence detection reagent into a working solution, and then add the ssDNA sample to be tested, then use the fluorescence microplate or Qubit Read with a fluorometer. The selectivity of this kit to single-stranded DNA is not higher than that to double-stranded DNA, but it has good tolerance to conventional pollutants such as proteins, salts, detergents, etc.

Features

Stable Signal: Combined with an exceptional dye and an upgraded, optimized buffer, it maintains stable fluorescence intensity at room temperature and exhibits strong applicability in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, or proteins, ensuring accurate quantification.

High Sensitivity: Detection concentration as low as 50pg/μL.

Rapid and Accurate: Features a simple operational workflow to achieve rapid quantification of oligonucleotides, primers, denatured DNA, and single-stranded circular DNA.

Applications

ssDNA quantification

MGI Circular library and DNB quantification

BS product quantification

Specifications

Components

Shipping and Storage

All the components are shipped with ice packs and can be stored at 2℃~8℃ away from light for 6 months. Please avoid repeated freeze-thawing.

Figures

Figure 1 Linearity Performance
Test Sample: ssDNA standards; Conclusion: Good linearity within 0-200ng

Figure 2 Comparison of measured values of different batches and original Thermophile products
The stability of Yeasen（Cat # 12645）three batches of products is good, which can accurately quantify different types of ssDNA samples, and consistent with the competitive products(Thermo Q10212)

Table 1 Good Contaminant Tolerance