Hieff NGS™ RNA Cleaner -12602ES
برای بزرگنمایی تصویر روی تصویر بچرخید برای بزرگنمایی روی تصویر کلیک کنید
Hieff NGS™ RNA Cleaner -12602ES
Hieff NGS™ RNA Cleaner -12602ES
Hieff NGS™ RNA Cleaner utilizes highly efficient magnetic beads combined with a proprietary buffer system to specifically bind RNA while effectively removing proteins, salts, and other impurities. It is ideal for purifying total RNA samples post-rRNA removal, in vitro transcribed RNA, RNA-labeled products, and synthetic RNAs. The purified RNA is compatible with RNA library preparation, RT-PCR, qRT-PCR, microarray analysis, Northern blotting, and RNA interference experiments. Features Premium Quality: High-quality raw materials ensure excellent recovery rates. RNA Integrity Protection: Optimized buffer system safeguards RNA integrity and prevents degradation. Broad Compatibility: Suitable for RNA library...

Frequently Bought Together

Hieff NGS™ RNA Cleaner -12602ES

YeasenSKU: 12602ES03
Size: 1 mL
قیمت:
$85.00
انبار:
در انبار

Hieff NGS™ RNA Cleaner utilizes highly efficient magnetic beads combined with a proprietary buffer system to specifically bind RNA while effectively removing proteins, salts, and other impurities. It is ideal for purifying total RNA samples post-rRNA removal, in vitro transcribed RNA, RNA-labeled products, and synthetic RNAs. The purified RNA is compatible with RNA library preparation, RT-PCR, qRT-PCR, microarray analysis, Northern blotting, and RNA interference experiments.

Features

Premium Quality: High-quality raw materials ensure excellent recovery rates.

RNA Integrity Protection: Optimized buffer system safeguards RNA integrity and prevents degradation.

Broad Compatibility: Suitable for RNA library preparation, in vitro transcription cleanup, RT-PCR, qRT-PCR, microarray analysis, and RNAi.

Specifications

Product Line

RNA clean Beads

Starting Material

RNA

Compatibility

RNA

Isolation Technology

Magnetic Bead

Final Product Type

RNA

For Use With (Application)

RNA celan up

Figures

1. High purification recovery efficiency

Figure 1. Recovery Efficiency of Different RNA Types 

Figure 1. Recovery Efficiency of Different RNA Types

Four total RNA samples (1.5 μg each) were diluted to 15 μL as unpurified controls. Another 1.5 μg of each sample was purified with 2.2× magnetic beads and eluted in 15 μL DEPC water. Concentrations were measured from 1 μL of each sample. Quality was checked by loading 1.5 μL on a 2100 Bioanalyzer. The 12602 beads showed over 80% recovery efficiency across all samples.

2. Higher RNA yield

Storage

This product should be stored at 2~ 8℃ for 12 months.

FAQ

There is no specific reference. You can first refer to the system in the user manual. For a single reaction with a 50 μl volume of sample, using 1.8 to 2 times the magnetic beads (100 μl magnetic beads) can purify 0.5 - 5 μg RNA.
Yes, small RNA can be recovered. The recovery rate has not been specifically tested. We usually use it for the purification of Total RNA.
For normal magnetic beads, when they stand still, the microbeads separate from the buffer and gather at the bottom of the bottle. Before using them, shake to mix evenly, and the microbeads are evenly distributed in the buffer. For abnormal magnetic beads, due to factors such as β-mercaptoethanol, DTT, and large pH differences, it affects the uniformity of the magnetic beads, causing the beads to become thick, aggregate into blocks, or have the phenomenon of sticking to the wall.
Storing at room temperature will have some impact on the recovery performance of the magnetic beads. It is not recommended to leave them at room temperature for too long. After use, it is recommended to store them at 4℃. The separation effect of PEG is easily affected by pH, temperature, etc.
12602: RNA purification magnetic beads; 12603: Complete mRNA capture magnetic beads for eukaryotes
1) Sample: The sample contains high levels of sugar or protein; the sample buffer and the magnetic bead buffer are incompatible;
2) Magnetic beads: The magnetic bead buffer has been contaminated.
3) Operation: The drying time should not be too long (generally, it should not exceed 5 minutes)
The product cannot be reused. The ethanol elution step will affect the performance of the surface-active groups on the magnetic beads.
It is not recommended. DNA magnetic beads for purifying RNA may cause RNA degradation. The system in RNA magnetic beads is more conducive to maintaining the stability of RNA structure and preventing its degradation.

Citations

Documents

Applications

You may also like

Inquiry

FAQ

The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.

Certain applications may require additional third-party intellectual property rights.

Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.