LZCap GG (3'Acm), (50mM) _ 10686ES
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LZCap GG (3'Acm), (50mM) _ 10686ES
LZCap GG (3'Acm), (50mM) _ 10686ES
LZCap GG (3'Acm) is a cap1 analog. The product is used for transcription with the initial sequence of 5'GG3', and the natural cap1 structure is produced by Cap1 transcription capping.  US patent approved. Components Cat No. Name Size 10686 LZCap GG (3'Acm) (50mM)  100 μL 1 mL Product Details Molecular Formula C35H48N16O25P4 (Free acid)  Molecular weight 1216.19 (Free acid) Concentration 50± 3 mM Purity HPLC ≥95% LZCap DNA Template Design LZCap GG(3'Acm) is suitable for GG-initiated sequences. As shown in the figure below, the SP6 promoter (underlined) followed by the GG sequence can effectively initiate transcription. Protocols 1． Thaw components required for the experiment on ice. 2． Add RNase free water and NTPs to reaction...

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LZCap GG (3'Acm), (50mM) _ 10686ES

ذخیره کنید $500.00
YeasenSKU: 10686ES60
Size: 100 μL
قیمت:
$500.00 $1,000.00
انبار:
در انبار

LZCap GG (3'Acm) is a cap1 analog. The product is used for transcription with the initial sequence of 5'GG3', and the natural cap1 structure is produced by Cap1 transcription capping.

US patent approved.

Components

Cat No.

Name

Size

10686

LZCap GG (3'Acm) (50mM)

100 μL

1 mL

Product Details

Molecular Formula

C35H48N16O25P4 (Free acid)

Molecular weight

1216.19 (Free acid)

Concentration

50± 3 mM

Purity

HPLC ≥95%

LZCap DNA Template Design

LZCap GG(3'Acm) is suitable for GG-initiated sequences. As shown in the figure below, the SP6 promoter (underlined) followed by the GG sequence can effectively initiate transcription.

Protocols

1． Thaw components required for the experiment on ice.

2． Add RNase free water and NTPs to reaction tube.

3． Heat LZCap GG(3'Acm) aliquot for 15 min at 60°C. Cool at room temperature for 5 min.

4． Immediately add LZCap GG(3'Acm) to tube and vortex to mix. Spin briefly to collect liquid.

5． Add 10x Transcription Buffer and vortex.

6． Add DNA template and SP6 Enzyme Mix.

7． Mix the prepared reaction solution, spin briefly, and incubate at 37°C for 2-3 hours. If the transcript length is less than 100nt, increase the reaction time to 4-8 h.

Component

Volume (μL)

Final concentration

RNase Free Water

Up to 20μL

/

ATP(100mM)

1

5mM

UTP(100mM)

1

5mM

CTP(100mM)

1

5mM

GTP(100mM)

0.2

1mM

LZCap GG(3'Acm) (50mM)

1.6

4mM

10×Transcription Buffer

2

1 ×

Linear DNA

1μg

50 ng/μL

SP6 Enzyme Mix

2

/

Final Volume

20μL

Notes:

1) The SP6 enzyme mix we recommended to used is MEGAscript® Kit（AM1330）.

2) LZCap GG(3'Acm) is suitable for SP6 promoter transcription vector with 5 ‘GG 3’initiated sequences, which needs to be considered when constructing the vector.

3) The reagents, consumables and containers used in the experiment are free of RNase contamination.

4) It is recommended to use a linearized DNA template for transcription.

5) When modified nucleotides were used in place of wild-type nucleotides, the final concentration of the reaction was unchanged.

6) Modified N1-Me-pUTP can be used in place of wild-type UTP. The modified N1-Me-pUTP reduces the immunogenicity of mRNA. Henovcom can also provide modified nucleotide N1-Me-pUTP (Cat. No.: HN1002).

7) If the PCR product is used as the transcription initiation DNA template, the amount of DNA template can be reduced by half.

Precautions

  1. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.
  2. For research use only.

Please contact us for more details about the LZCap licensing.

Storage

This product could be stored at -25~-15 ℃ for two years.

FAQ

Enzymes have a relatively "specific" recognition of substrates. Therefore, when designing a new cap structure, on one hand, we need novel structural modifications for patent purposes, but on the other hand, we strive to maintain similarity with natural/known structures as much as possible. The natural structure has a ribose 3' OH, which can be modified (e.g., methylation). Based on this consideration, we chose to add a carbon at the 3' position for patent novelty, followed by an NH to mimic the hydrogen bonding of OH, and then an acetyl group to reduce the basicity of NH and enhance its hydrogen bonding capability. The activity of LzCap is better than methylated natural cap, possibly due to increased hydrogen bonding. Compared to the methyl and methoxy groups, the acetyl amino group may also increase van der Waals interactions between the substrate (cap) and the initiating factor (enzyme).
The acetyl amino group is already sufficiently stable. It is much more stable than the 7-methylated position and the phosphodiester bond, which are the least stable parts of the cap.

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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.

Certain applications may require additional third-party intellectual property rights.

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