Pseudouridine-5-triphosphate solution is one of the most commonly used modified nucleoside triphosphates. It is mostly used as the reaction substrate or coenzyme of enzymes, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. The modified mRNA containing pseudouridine has better nuclease stability and translation characteristics and reduces its immunogenicity. It has a wide range of applications in the field of therapy and diagnosis.
This product is produced in accordance with GMP process requirements and provided in liquid form.

Feature

Validated, product-specific processes and analytical methods

Product-specific stability

Documentation follows applicable GMP guidelines

AOF production process and raw materials (TSE & BSE)

Nitrosamine statement

Regulatory support documents available

Large-scale production

The increased IVT yield and the decreased dsRNA content under the optimized Tris NTP reaction system

Application

RNA synthesis and amplification

Building block for in vitro transcription

Specification

CAS No.	1175-34-4 (free acid)
Molecular formula	C9H15N2O15P3 (free acid)
Molecular weight	484.14 g/mol (free acid)
Purity	≥ 99%
Content	100 mM ± 3 mM
Structure

Component

Figures

• Increased the IVT yield

Figure 1. The IVT yield was significantly increased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system.

• Decreased the dsRNA content

Figure 2. The content of dsRNA was significantly decreased under the optimized Tris NTP reaction system, compared with the sodium NTP reaction system. The content of dsRNA was detected by Dot Blot method.