Pseudouridine-5-triphosphate, trisodium salt solution is one of the most commonly used modified nucleoside triphosphates. It is mostly used as the reaction substrate or coenzyme of enzymes, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. The modified mRNA containing pseudouridine has better nuclease stability and translation characteristics, and reduces its immunogenicity. It has a wide range of applications in the field of therapy and diagnosis.
This product is produced in accordance with GMP process requirements and provided in liquid form.

Feature

Validated, product-specific process and analytical methods

Product-specific stability

Documentation follows applicable GMP guidelines

AOF production process and raw materials (TSE & BSE)

Nitrosamine statement

Regulatory support documents available

Large-scale production

Nucleotide in the multiple salt form（Na⁺, Tris etc) always available to meet different downstream application needs

Application

Modified RNA synthesis and amplification

Building block for in vitro transcription

Specification

CAS No	1175-34-4 (free acid)
Formula	C9H12N2Na3O15P3
Molecular Weight	550.10 g/moL
Purity (HPLC)	≥ 99%
Content	100 mM ± 3 mM
Structure

Component

Figures

• Standard RNA Synthesis

Figure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.

The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.

• Capped RNA Synthesis

Figure 2. Synthesis of capped RNA in vitro.

The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 2A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 2B.