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Proteinase K is a serine protease with wide cleavage activity, which can cleave the carboxyl-terminal peptide bond of aliphatic and aromatic amino acids. Its relative molecular weight is about 29.3 kDa. Proteinase K is widely used in the preparation of chromosomal DNA for pulse electrophoresis, Western blotting, and the removal of a nuclease from DNA and RNA preparation. Denaturant such as SDS (1%) can improve its activity. In addition, the common working concentration is 50-100 μg/mL, and the specific working concentration is according to whether the buffer contains SDS, urea, pH, temperature, and other factors.
Applications
Genomic DNA extraction
Digestion and removal of the enzyme
Features
Derived from a genetically engineered yeast strain.
Free of RNase and DNase contamination.
Maintains stable activity in the presence of urea and SDS.
Active across a broad pH range (pH 4.0 – 12.0), with optimal activity between pH 7.5 and 9.0.
Specifications
| Source | Yeast |
| Specific Activity | ≥30 U/mg |
| Unit Definition | One unit is defined as the amount of proteinase K that will liberate 1μmoL tyrosine per minute at 37℃ @ pH 7.5. |
| Purification | Ni column affinity purification |
| Reaction Condition | The reaction buffer should be incubated at 37℃ -70℃ for 1h, and the recommended reaction temperature is 70℃. |
| Inactivation method | The reaction system can be inactivated by adding PMSF or DFP inhibitor and partially inactivated by incubation at 65℃ for 10-15 min. |
| Activity Range | It has activity in a wide range of pH (4.0-12.0) and the optimal pH range is 7.5-9.0. |
| RNase and DNase | None |
Components
| Components No. | Name | 10401ES60 | 10401ES80 | 10401ES90 | 10401ES98 |
| 10401 |
Proteinase K Powder（RNase-Free, DNase-Free）
|
100 mg | 1 g | 5 g | 100 g |
Storage
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