Hieff™ Taq DNA polymerase is a thermostable recombinant wild type DNA polymerase expressed by Thermus aquaticus with a molecular weight of 94 KDa. It has 5'→3' polymerase activity and 5'→3' exonuclease activity but without 3'→5' exonuclease activity. The amplified product has 3'- dA and can be directly used for TA cloning.
Features
5'→3' DNA polymerase activity
5'→3' exonuclease activity
Hieff™ Taq DNA polymerase is an ideal tool for standard PCR with templates of 5 kb or less
Applications
Genotyping, colony PCR and other conventional PCR
Specifications
| Polymerase | Taq DNA polymerase |
| Molecular Weight | 94 KDa |
| Unit Definition | Using the activated DNA of salmon sperm as template/primer, the activity is defined as one active unit (U) when 10 nmol of total nucleotide was ingested as acid insoluble substance at 74℃ for 30 min. |
Components
| Components No. | Name | 10101ES80 (1, 000 U) | 10101ES92 (10, 000 U) |
| 10101-A | 10×Taq Buffer (Mg2+ Free) | 4×1 mL | 4×10 mL |
| 10101-B | 25 mM MgCl2 | 2×1 mL | 2×10 mL |
| 10101-C | Hieff™ Taq DNA Polymerase (5 U/μL) | 200 μL | 2×1 mL |
Storage
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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