Adeno-associated viruses (AAV) and lentiviruses (LV) are widely used in cell and gene therapy due to their high safety and efficacy profiles. These vectors are typically produced on a large scale in HEK293 cells using polyethylenimine (PEI)-mediated transient transfection methods. YEASEN Biotech, leveraging its strong R&D capabilities, offers a range of high-quality PEI-based transfection reagents, with specific modifications to PEI to enhance its performance in particular application scenarios. To address the challenge of producing sufficient quantities of AAV for clinical development, YEASEN Biotech has developed a novel transfection reagent, PEI-AAV, specifically designed for large-scale AAV production in suspension systems and suitable for use in large bioreactors.
PEI-AAV- Suspended System AAV Virus Production Transfection Reagent.
Economical and efficient: In the suspension system, higher AAV virus titers can be produced, and higher virus yields can still be achieved with less DNA.
Operationally convenient: The complex volume can be reduced to 1%, and the transfection complex stability can last up to 6 hours, meeting the needs of large-scale production.
Easy to scale up: Offers both research-grade and GMP-grade products, meeting the needs from small-scale processes to large-scale production.
Broad applicability: Capable of producing higher virus titers for a variety of AAV serotypes.
Achieves extremely high virus titers for various AAV serotypes.
PEI-AAV transfection reagent, compared to PEI and other competitors, can significantly increase AAV production when producing AAV2/AAV5/AAV8/AAV9 in suspended 293F cells, with virus yields markedly higher than those of competitors; it also has a high transfection efficiency for different serotypes.
Higher AAV titers with less DNA usage.
Compared to Suppliers, PEI-AAV transfection reagent can enhance virus production while using less DNA, significantly reducing production costs in large-scale manufacturing.
Provides flexibility for large-scale production.
The transfection complex volume can be reduced to 1%, and it has a stability of up to 6 hours. These characteristics together provide great convenience and cost-effectiveness for the large-scale preparation of AAV viral vectors.
Commitment to Quality and Compliance
As a global supplier of biopharmaceutical raw materials, YEASEN Biotech is dedicated to delivering both R&D-grade and GMP-grade PEI transfection reagents. Our products are manufactured under strict GMP standards and the ISO 13485 quality system, ensuring consistency, reliability, and regulatory compliance.
YEASEN has successfully supported numerous customers in advancing projects through IND submissions, providing not only high-quality reagents but also regulatory documentation and audit support. By combining technical expertise with comprehensive customer service, YEASEN enables partners to accelerate development while reducing costs.
With its high efficiency, scalability, and cost-saving advantages, YEASEN’s PEI-AAV transfection reagent is an ideal choice for researchers and manufacturers aiming to achieve robust AAV production—from early R&D to clinical-scale manufacturing in large bioreactors.
Product List
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Product Name |
Product Specifications |
Product number |
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1 mL /10 mL /100mL |
40823ES03/10/60 |
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10 mL /100 mL/1L |
40824ES10/60/80 |