1.Background Introduction
Streptavidin is a biotin-binding protein derived from Streptomyces avidinii. Its non-specific binding is much lower than that of avidin, and compared to egg white avidin, which has a net positive charge at neutral pH and contains about 7% carbohydrates, streptavidin has more favorable chemical properties such as almost no net charge at neutral pH and no carbohydrates. Therefore, it is widely used as an alternative to avidin.
2.Product Features
Tetramer structure: Streptavidin exists as a homotetramer with a size of 66KDa;
High affinity: Each molecule of streptavidin can highly specifically bind to four molecules of biotin, with an extremely strong affinity between them;
Wide Applications: The dissociation constant of the streptavidin-biotin complex is in the nanomolar range, a property commonly used in molecular biology applications; In most applications, it can be used interchangeably with antibody proteins;
High-Quality Extraction: Obtained by purification from Streptomyces avidinii;
Figure 1. Streptavidin
Product Application
Application 1: Purified resin with streptavidin
1)Application principleBased on the interaction between streptavidin and biotin, streptavidin is highly cross-linked to 6% agarose, and the unique preparation process gives it higher physicochemical properties, allowing it to withstand higher pressures. It can achieve purification of the target protein at relatively high flow rates, making it more suitable for large-scale industrial protein purification.
Figure 2. Purification principle of streptavidin resin
2）Yeasen is committed to providing you with high-efficiency products to purify more perfect proteins for you.
Figure 3. Protein purified by streptavidin resin
3）Frequently Asked Questions|
Question |
Possible reason |
Recommended solution |
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The back pressure of the column is too high |
Packing is blocked |
Clean the resin. |
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Lysis buffer contains small solid particles, it is recommended to filter with 0.22μm or 0.45μm filter membrane before loading, or centrifuge to remove. |
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The sample is too viscous. |
Sample contains high concentration of nucleic acids, extend lysis time until viscosity is reduced, or add DNase I (final concentration of 5 μg/ml), Mg2+ (final concentration of 1 mM), incubate on ice for 10-15 minutes |
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Buffer is too viscous |
Organic reagents or protein stabilizing reagents (such as glycerol, etc.) may cause increased back pressure, reduce operating flow rate. |
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No-purpose protein in elution component |
Excessive cleavage denatures the protein of interest |
Use mild lysis conditions. experimental conditions are based on experience. |
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The target protein aggregates to produce precipitation |
Add DTT to pre-cell lysis solution at a final concentration of 1-10 mM. |
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The target protein is not completely eluted |
The elution volume is too small |
Increase the eluent volume and decrease the elution flow rate. |
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The pH of the elution buffer changes |
Use freshly prepared eluent |
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The eluent incubation time is not enough |
Increase the incubation time of the elution buffer with the gel. |
Application 2: Fluorescent group and enzyme conjugate labeled streptavidin.
Figure 4. Different Conjugated Streptavidins
1）Enzyme Conjugated Streptavidins- Horseradish Peroxidase (HRP) Conjugated Streptavidin
Ⅰ.Produced by high-purity streptavidin and horseradish peroxidase through a special conjugation technique;
Ⅱ.Maintains the high specific activity of peroxidase. Applications: Immunohistochemistry (IHC), Immunocytochemistry (ICC), In situ Hybridization (ISH), etc.
Recommended Concentration: 0.01-0.1 µg/mL for WB (chemiluminescence method); 1-2µg/mL for ELISA, HC/ICC, WB (substrate color development)
- Alkaline Phosphatase (AP) Conjugated Streptavidin
Ⅰ.Suitable for solid-phase analysis, tissue/cell staining systems, and blot applications, with specific covalent linkage;
Ⅱ.The conjugate is stable and highly active.
Applications: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Enzyme-Linked Immunospot Assay (ELISPOT), Enzyme-Linked Immunosorbent Assay (ELISA), In situ Hybridization (ISH), and blot applications.
Recommended Concentration: 1-2 µg/mL for ELISA, IHC/ICC; 0.1-1 µg/mL for WB
2）Fluorophore Conjugated Streptavidins- Fluor® Fluorescein Conjugated Streptavidin
Ⅰ.Recommended for routine immunofluorescence;
Ⅱ.Highly purified with very low non-specific binding characteristics;
Ⅲ.High affinity for biotin;
Applications: Immunofluorescence (IF), In situ Hybridization (ISH), Flow Cytometry (Flow Cyt).
Recommended Concentration: 0.5-2 µg/mL (IF)
- DyLight™ Dye Conjugated Streptavidin
Ⅰ.Can be used for detecting biotinylated second antibodies and other large molecules in immunofluorescence, in situ hybridization, or flow cytometry applications;
Ⅱ.Highly purified with very low non-specific binding characteristics;
Ⅲ.Extremely high affinity for biotin;
Ⅳ.High photostability, pH insensitive, and brighter fluorescence;
Applications: Immunofluorescence (IF), In situ Hybridization (ISH), Flow Cytometry (Flow Cyt).
Recommended Concentration: 0.5-2 µg/mL (IF)
Product Information
|
Product Series |
Product Number |
Product Name |
Specification |
|
Streptavidin |
35099ES |
Streptavidin (Powder) |
100 mg/1 g |
|
35101ES |
Streptavidin (5mg/ml) |
1 mg/5 mg/10 mg/ 100 mg/1 g |
|
|
Enzyme Conjugated Streptavidins |
35105ES |
HRP Streptavidin |
100 μL |
|
35106ES |
AP Streptavidin |
100 μL |
|
|
Fluorescently labeled streptavidin |
35102ES |
DyLight 405™ Streptavidin |
100 μL |
|
35103ES |
YSFluorTM 488-conjugated Streptavidin |
100 μL |
|
|
35104ES |
YSFluorTM 647-conjugated Streptavidin |
100 μL |
|
|
35107ES |
YSFluorTM 584-conjugated Streptavidin |
100 μL |
|
|
35108ES |
Cy3-conjugated Streptavidin |
1 mg |
|
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Streptavidin Purification Series |
20512ES |
Streptavidin Agarose Resin 6FF |
5 mL/5mL×5/100 mL |
|
20513ES |
BiotSep Streptavidin 6FF Chromatography Column, 1ML |
1 mL/1mL×5 |
|
|
20514ES |
BiotSep Streptavidin 6FF Chromatography Column, 5ML |
5 mL/5 mL×5 |