Antibody Capture Tool, Easily Handle IP/COIP

——Protein A/G Agarose or Magnetic Beads

Protein A and Protein G are the most commonly used proteins for antibody affinity purification and immunoprecipitation. Protein A/G agarose or magnetic beads covalently couple Protein A and G to the medium surface, featuring stronger binding capacity, a broader binding range, and higher practicality.

rProtein A/G Agarose Resin

Protein A/G MagBeads

Matrix

Highly cross-linked 4% agarose microspheres

Polymeric magnetic microspheres

Particle Size

45-165 µm

1 µm

Capacity

>20 mg hIgG/mL resin

>0.7 mg hIgG/mg magnetic beads

Advantages

Easy to use, no auxiliary equipment required

Simple operation

Large pore size, strong binding force

Small diameter, good kinetics

High yield of target protein

Smooth surface, minimal bead adsorption, low background, less antibody consumption

Disadvantages

Porous and prone to adsorption (pre-clear required)

Convenient to use with a magnetic rack

Product Applications

1.Purification of Antibodies

    A single step of purification can isolate high-purity antibodies from serum or ascites samples.

    2.Immunoprecipitation (IP)

      Enrichment of a specific protein to study its characteristics (such as post-translational modifications).

       

      3.Co-Immunoprecipitation (Co-IP)

      Enrichment of a specific protein to study other proteins that bind to it (i.e., protein-protein interactions, protein complexes, etc.).

      Operation Procedure

      Test Data

      1.Purification of antibodies from rabbit serum using rProtein A/GAgarose Resin.

      Figure 1. Electrophoresis result of antibody purification using rProtein A/G Agarose Resin

      2.Use of Protein A/G Magbeads for Co-IP: Specific antibodies against p65 and cyclin-dependent kinase 9 (CDK9) are used for immunoprecipitation analysis of extracted total cellular protein.

      Figure 2. Immunoprecipitants were then subjected to western blot analysis using specific antibodies against CDK9 and p65.

      Product Information

      Product Name

      Product Number

      Specification

      rProtein A/G Agarose Resin

      36403ES08/25/60

      5 mL/25 mL/100 mL

      Protein A/G Magbeads

      36417ES03/05

      1 mL/5 mL

      Related Products Order

      Product Name

      Product Number

      Specification

      SuRi rProtein A Agarose Resin

      36401ES08/25/60

      5 mL/25 mL/100 mL

      rProtein G Agarose Resin

      36405ES08/25/60

      5 mL/25 mL/100 mL