The Whole Transcriptome Research
[Whole Transcriptome Sequencing - Research on the Targeted Regulation and Interaction Relationship between Non-coding RNA and mRNA]
Whole transcriptome high-throughput sequencing adopts the ribosome-depleted strand-specific library construction method and the small fragment enrichment screening library construction method, which can achieve the library construction, sequencing, information analysis ,joint analysis and ceRNA, etc. of coding RNA and non-coding RNA, So in that way it can obtain the whole RNA transcript data information related to specific biological processes (such as development, disease, etc.) quickly, comprehensively and accurately. Through data analysis, the research on biological regulation mechanism can be extended to a three-dimensional model combining "network and multi-level", which is helpful to comprehensively interpret biological phenomena.
There are two ways of whole transcriptome sequencing, one is LncRNA-Seq + Small RNA, and the other is LncRNA-Seq + Small RNA + CircRNA, which can obtain more comprehensive information of CircRNA.
1.Whole transcriptome sequencing with two libraries: LncRNA-Seq + Small RNA
2.Whole transcriptome sequencing with three libraries: LncRNA-Seq + Small RNA + CircRNA
Whole transcriptome Raw Material Solution
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Classification |
Animal Category |
Plant Category |
Bacterial and Fungal Category |
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Sample Type |
Animal Tissue/Cell |
Cell |
Whole Blood |
Plant Tissue |
Fungal Tissue |
Cultured Bacteria and Fungi |
|
RNA Extraction |
Magnetic beads method: 18605/18607/18606; Column method: 19221/19211; Trizol method: 10606/19202 |
Magnetic beads method: 18600/18604; Column method: 19231 |
Column method: 19241; Trizol method: 19201 |
Magnetic beads method: 18534/18535/18537; Column method: 19291ES/19292ES |
Magnetic beads method: 18534/18535/18537; Column method: 19291ES/19292ES |
Column method: 19301 (fungi need to add Lysozyme 10403) |
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miRNA Extraction |
Column method: 19331 |
Column method: 19331 |
Column method: 19332 |
Column method: 19331 |
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— |
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miRNA Library Construction |
To be expected |
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rRNA Removal |
RNase H enzymatic removal: 12257; 3-minute rapid removal: 12258 (human source removal); Magnetic beads method removal: 12266 (mammalian universal type)/12267 avian universal type/12268 domestic chicken/12269 zebrafish/12270 Drosophila /12275 oyster/12278 planarian/12285 bee/12286 golden orb-web spider/12289 tick/12290 Aedes albopictus/12287 nematode |
RNase H enzymatic removal: 12257; 3-minute rapid removal: 12258 (human removal, suitable for pathogen) |
RNase H enzymatic removal, including globin removal: 12257 + 12806; RNase H enzymatic removal of globin: 13572; 3-minute rapid removal: 12258 + 12260 |
RNase H enzymatic removal: 12254; Magnetic beads method removal: 12262 angiosperm |
Magnetic beads method removal: 12271 fungal universal type |
Magnetic beads method removal: 12264 bacterial universal type/12265 prokaryotic universal type |
|
Library Construction |
Non-premixed RNA library construction kit: 12308 dual-mode RNA library construction kit (compatible with Illumina and MGI); Premixed RNA library construction kit: 12310 dual-mode RNA library construction kit (compatible with Illumina and MGI)/12340 (without actinomycin D, dUTP)/12341 (without actinomycin D, dTTP) |
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LncRNA Research
Long non-coding RNA (LncRNA) is a kind of non-coding RNA with unique regulatory function that has attracted extensive attention in recent years. The length of LncRNA is more than 200nt, it does not encode proteins, and its conservation among species is poor. Some of its sequence structures are similar to mRNA (containing polyA tail and alternative splicing), while others do not have these characteristics. At present, the generation and functional mechanism of LncRNA are not yet fully understood, however, many LncRNAs with important biological functions (such as cancer occurrence, X chromosome silencing and the formation of complex regulatory networks with other regulatory factors) have been discovered.
LncRNA Sequencing Technical Route
LncRNA Sequencing Raw Material Solution
|
Classification |
Animal Category |
Plant Category |
Bacterial and Fungal Category |
|||
|
Sample Type |
Animal Tissue/Cell |
Cell |
Whole Blood |
Plant Tissue |
Fungal Tissue |
Cultured Bacteria and Fungi |
|
RNA Extraction |
Magnetic beads method: 18605/18607/18606; Column method: 19221/19211; Trizol method: 10606/19202 |
Magnetic beads method: 18600/18604; Column method: 19231 |
Column method: 19241; Trizol method: 19201 |
Magnetic beads method: 18534/18535/18537; Column method: 19291ES/19292ES |
Magnetic beads method: 18534/18535/18537; Column method: 19291ES/19292ES |
Column method: 19301 (fungi need to add lywallzyme 10403) |
|
rRNA Removal |
RNase H enzymatic removal: 12257; 3-minute rapid removal: 12258 (human source removal); Magnetic beads method removal: 12266 (mammalian universal type)/12267 avian universal type/12268 domestic chicken/12269 zebrafish/12270 Drosophila /12275 oyster/12278 planarian/12285 bee/12286 golden orb-web spider/12289 tick/12290 Aedes albopictus/12287 nematode |
RNase H enzymatic removal: 12257; 3-minute rapid removal: 12258 (human removal, suitable for pathogen) |
RNase H enzymatic removal, including globin removal: 12257 + 12806; RNase H enzymatic removal of globin: 13572; 3-minute rapid removal: 12258 + 12260 |
RNase H enzymatic removal: 12254; Magnetic beads method removal: 12262 angiosperm |
Magnetic beads method removal: 12271 fungal universal type |
Magnetic beads method removal: 12264 bacterial universal type/12265 prokaryotic universal type |
|
Library Construction |
Non-premixed RNA library construction kit: 12308 dual-mode RNA library construction kit (compatible with Illumina and MGI); Premixed RNA library construction kit: 12310 dual-mode RNA library construction kit (compatible with Illumina and MGI)/12340 (without actinomycin D, dUTP) |
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Eukaryotic Transcriptome Research
Eukaryotic transcriptome research uses RNA-seq technology to obtain the total mRNA that can be transcribed by specific cells in a certain functional state. Then, the downloaded raw data is spliced and assembled, the gene expression level is counted, and differential analysis and functional enrichment analysis are carried out. It can not only obtain most of the gene information of the species, but also study the gene expression difference and functional pathway change at the overall level, and reveal the molecular mechanism of specific biological processes.
Eukaryotic Transcriptome Sequencing Technical Route
Eukaryotic Transcriptome Sequencing Raw Material Solution
|
Classification |
Animal Category |
Plant Category |
Fungal Category |
|||
|
Sample Type |
Animal Tissue/Cell |
Cell |
Whole Blood |
Plant Tissue |
Fungal Tissue |
Cultured Fungi |
|
RNA Extraction |
Magnetic beads method: 18605/18607/18606; Column method: 19221/19211; Trizol method: 10606/19202 |
Magnetic beads method: 18600/18604; Column method: 19231 |
Column method: 19241; Trizol method: 19201 |
Magnetic beads method: 18534/18535/18537; Column method: 19291ES/19292ES |
Magnetic beads method: 18534/18535/18537; Column method: 19291ES/19292ES |
Column method: 19301 (add lysozyme 10403) |
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mRNA Capture |
12629 mRNA Isolation Master Kit |
12629 mRNA Isolation Master Kit |
12629 mRNA Isolation Master Kit |
12629 mRNA Isolation Master Kit |
12629 mRNA Isolation Master Kit |
12629 mRNA Isolation Master Kit |
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Library Construction |
Non-premixed RNA Library Kit: 12308 Dual-Mode RNA Library Kit (Illumina and MGI compatible) Non-premixed RNA Library Kit with mRNA Capture: 12309 Dual-Mode RNA Library Kit (Illumina and MGI compatible) Premixed RNA Library Kit: 12310 Dual-Mode RNA Library Kit (Illumina and MGI compatible)/12340 (without Actinomycin D, dUTP) |
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circRNA Research
[circRNA Sequencing - Research on How the Differential Expression of circRNA Affects Biological Processes]
Circular RNA (circRNA) is a special type of non-coding RNA analysis. Sequencing is carried out using a sequencing platform to conduct accurate circRNA identification and analysis of the source genes for samples with a reference genome. circRNA sequencing is increasingly widely used in medical and agricultural research fields. Based on high-throughput sequencing and relying on mainstream databases and circRNA identification software, the circRNA information of the project species is analyzed to deeply explore the important functions of circRNA in transcriptional regulation.
circRNA Research Raw Material Solution
|
Classification |
Animal Category |
Plant Category |
Bacterial and Fungal Category |
|||
|
Sample Type |
Animal Tissue/Cell |
Cell |
Whole Blood |
Plant Tissue |
Fungal Tissue |
Cultured Bacteria and Fungi |
|
RNA Extraction |
Magnetic beads method: 18605/18607/18606; Column method: 19221/19211; Trizol method: 10606/19202 |
Magnetic beads method: 18600/18604; Column method: 19231 |
Column method: 19241; Trizol method: 19201 |
Magnetic beads method: 18534/18535/18537; Column method: 19291ES/19292ES |
Magnetic beads method: 18534/18535/18537; Column method: 19291ES/19292ES |
Column method: 19301 (fungi need to add lysozyme 10403) |
|
rRNA Removal |
RNase H enzymatic removal: 12257; 3-minute rapid removal: 12258 (human source removal); Magnetic beads method removal: 12266 (mammalian universal type)/12267 avian universal type/12268 domestic chicken/12269 zebrafish/12270 Drosophila /12275 oyster/12278 planarian/12285 bee/12286 golden orb-web spider/12289 tick/12290 Aedes albopictus/12287 nematode |
RNase H enzymatic removal: 12257; 3-minute rapid removal: 12258 (human removal, suitable for pathogen) |
RNase H enzymatic removal, including globin removal: 12257 + 12806; RNase H enzymatic removal of globin: 13572; 3-minute rapid removal: 12258 + 12260 |
RNase H enzymatic removal: 12254; Magnetic beads method removal: 12262 angiosperm |
Magnetic beads method removal: 12271 fungal universal type |
Magnetic beads method removal: 12264 bacterial universal type/12265 prokaryotic universal type |
|
Linear RNA Digestion |
14606 RNase R to remove linear RNA molecules |
14606 RNase R to remove linear RNA molecules |
14606 RNase R to remove linear RNA molecules |
14606 RNase R to remove linear RNA molecules |
14606 RNase R to remove linear RNA molecules |
14606 RNase R to remove linear RNA molecules |
|
Library Construction |
Non-premixed RNA library construction kit: 12308 dual-mode RNA library construction kit (compatible with Illumina and MGI); Premixed RNA library construction kit: 12310 dual-mode RNA library construction kit (compatible with Illumina and MGI)/12340 (without actinomycin D, dUTP) |
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Metatranscriptome
[Metatranscriptome - Capturing the Dynamic Changes of Active Microorganisms in the Microecological Microenvironment]
Metatranscriptome sequencing:studies the transcription and regulation rules of all genomes in a specific environment at the overall level. Taking all RNA in the microecological microenvironment as the research object, it combines high-throughput sequencing to study the changes of complex microbial communities at the transcriptional level and mine the active genes that play a role.
Metatranscriptome Research Raw Material Solution
|
Sample Type |
Cultured Bacteria and Fungi |
Clinical Samples |
|
RNA Extraction |
Column method: 19301 (fungi need to add lywallzyme 10403) |
Column method: 19321 (viral DNA/RNA extraction); Magnetic beads method: 18521 (viral DNA/RNA extraction); 18306 (pathogen DNA/RNA extraction) |
|
rRNA Removal |
3-minute rapid removal: 12258 (human source removal) |
3-minute rapid removal: 12258 (human source removal) |
|
Library Construction |
Option 1. Total RNA library construction: 12308ES dual-mode RNA library construction kit (compatible with Illumina and MGI); Option 2. cDNA enzymatic digestion library construction: 13488ES cDNA synthesis module + 12316ES rapid enzymatic digestion library construction kit |
Option 1. Total RNA library construction: 12308ES dual-mode RNA library construction kit (compatible with Illumina and MGI); Option 2. cDNA enzymatic digestion library construction: 13488ES cDNA synthesis module + 12316ES rapid enzymatic digestion library construction kit |
Product Recommendation
|
Product Name |
Catalog Number |
Specification |
|
Hieff NGS TM EvoMax RNA Library Prep Kit（dUTP） |
12340ES24/96 |
24 T/ 96 T |
|
Hieff NGSTM EvoMax RNA Library Prep Kit(dNTP) |
12341ES24/96 |
24 T/ 96 T |
|
Hieff NGSTM Ultima Dual-mode RNA Library Prep Kit （Illumina and MGI) |
12308ES24/96 |
24 T/ 96 T |
|
Hieff NGSTM Ultima Dual-mode RNA Library Prep Kit(Premixed version) |
12310ES24/96 |
24 T/ 96 T |
|
Hieff NGSTM mRNA Isolation Master Kit V2 |
12629ES24/96 |
24 T/ 96 T |
|
Hieff NGSTM MaxUp Human rRNA Depletion Kit(rRNA & ITS/ETS) |
12257ES24/96 |
24 T/ 96 T |
|
Hieff NGSTM MaxUp rRNA Depletion Kit(Plant) |
12254ES24/96 |
24 T/ 96 T |
|
Hieff NGSTM One-Step rRNA Removal Kit(Human,100-1,000 ng) |
12258ES24/96 |
24 T/ 96 T |
|
Hieff NGSTM MagSP rRNA Removal Kit (Bacteria(G- and G+)) with purification beads |
12264ES24/96 |
24 T/ 96 T |