Why Traditional qPCR&RT-qPCR Reagents Are Holding You Back
Conventional qPCR and RT-qPCR reagents often require multiple manual preparation steps—mixing primers, probes, enzymes, and buffers separately. This not only increases setup time, but also leaves room for human error. In fact, studies show that manual prep can result in error rates as high as 8–12%, depending on the operator’s experience and pipetting accuracy.
- Other limitations of traditional reagents include:
- Higher variability (CV ≥ 15%) leading to inconsistent results
- Lower sensitivity, with detection limits typically around 1–10 copies
- Longer workflows, often requiring 2–4 hours including pre-treatment
- Limited application to controlled lab settings with trained personnel
- High reagent waste and labor costs, with utilization rates below 80%
All-in-One Power: Fully Pre-Mixed, Fully Optimized
---Yeasen's Dual Guarantee: Premium Materials + Innovative Process
At Yeasen, every fully pre-mixed qPCR and RT-qPCR reagent is built on two pillars: top-quality raw materials and cutting-edge production technology. Our R&D team rigorously selects and tests each component to ensure purity, stability, and compatibility, while our advanced manufacturing process guarantees consistent, high-performance mixes—batch after batch.
This dual approach empowers researchers and diagnostic labs with ready-to-use solutions that simplify workflows, reduce errors, and deliver reliable, reproducible results. With Yeasen, you’re not just saving time—you’re gaining confidence in every run.
Figure 1. All-in-One vs. Traditional qPCR/RT-qPCR Reagents
1. Fully Pre-Mixed DNA Mix
(A) . Hieff UniconTM Pro Universal TaqMan Multiplex qPCR Mix (One-Tube, UDG Plus)(Cat#16710)
1)Product propertity
2)Ultra-Sensitive Amplification Performance
Figure 2. Reliable low-copy detection in multiplex qPCR using All-in-One premixed reagent
In a 4-plex system, Cat#16710ES reliably detected ultra-low concentration samples (250 copies/mL), demonstrating excellent sensitivity with a detection limit of at least 250 copies/mL.
3) Excellent Stability of All-in-One Premix
Figure 3. Stability evaluation of All-in-One premixed reagent
Yeasen’s premix (Cat#16710ES) was tested under 4°C, 37°C (7 days), and 50 freeze-thaw cycles. Compared to the -20°C control, ΔCt values stayed within ±0.5 and fluorescence deviations under 15% across four multiplex qPCR systems (3 quadruplex, 1 triplex), confirming its strong stability.
(B).Ultra-Clean, Low DNA residue--Hieff UniconTM Pure Pro U+ qPCR Mix(One Tube)(Cat#16713)
1)Ultra-Clean Production Control
Figure 4. Ultra-Clean reagent production and quality control workflow
2) Ultra-Clean (Low Residual Contamination, Low host DNA residue)
Figure 5. Residual bacterial DNA and huaman DNA test
NTC (no template control) qPCR using Yeasen one-tube premix (Cat#16713ES) and conventional reagents was tested on E. coli, P. aeruginosa, MecA, and human DNA systems. No amplification was detected in 48-well NTCs with Yeasen premix, confirming ultra-low background bacterial and human DNA contamination.
3) Outstanding Fully Premix Stability
Figure 6.Thermal accelerated stability test(37℃ 14days)
4)Compatibility with rapid protocols
Figure 7. Rapid Protocol Performance Validation using theHieff Unicon™ Pure Pro U+ qPCR Mix(One Tube, low DNA)（Cat#16713）
Seven gradient samples (1–10⁶ copies/μL) were tested using both standard (66 min) and rapid (30 min) protocols on ABI Q5. The rapid protocol showed >99% efficiency, ΔCt within ±0.5, fluorescence deviation within ±10%, and typical S-shaped amplification curves.
2. Fully Pre-Mixed RT-qPCR Mix
HifairTM Superpro Ⅱ One Step RT-qPCR Master Mix(Cat#11899)
1）Excellent amplification performance
Figure 8. Comparison of Yeasen’s fully premixed RT-qPCR reagent (Cat#11899), conventional reagent (Cat#13650), and competitor T brand for detecting Influenza A and B at 2000 and 200 copies/mL.
Yeasen’s reagent matched the conventional reagent in performance and showed 1–2 cycle earlier Ct values than T brand, indicating better sensitivity at low target levels.
2）High Sensitivity
Figure 9. RT-qPCR targeting Influenza A and Influenza B was performed using the HifairTMSuperpro Ⅱ One Step RT-qPCR Master Mix(Cat11899).
The reagent successfully detected all low-concentration Influenza A and B templates (8/8), demonstrating excellent sensitivity.
3）High-Stability One-Tube Premix System
(A)Stability Test at 4 °C for 7 Days
(B). Stability Test at 37 °C for 5 Days
Figure 10.Stability evaluation of fully premixed RT-qPCR reagent(Cat#11899) with primers and probes added, stored at 4 °C for 7 days(A)and 37 °C for 5 days(B).
(A) 4 °C, 7-Day Stability: Yeasen premix with primers/probes showed stable Ct and fluorescence values for Influenza A/B (2000 & 200 copies/mL), with better performance than competitor T after 7 days at 4 °C.
(B) 37 °C, 5-Day Accelerated Stability: Yeasen premix remained stable after 5 days at 37 °C, with no Ct shift when detecting RBP (5000 copies/mL) and RSV (500 copies/mL).
Product list
|
Category |
Name |
Cat. No. |
|
|
Probe qPCR Kit |
"one-tube" fully premixed reagents |
Hieff UniconTM Pro Universal TaqMan Multiplex qPCR Mix (One-Tube, UDG Plus) |
16710ES |
|
"one-tube" fully premixed reagents, ultra-low DNA residue |
16713ES |
||
|
Lyo-Ready qPCR reagent |
11893ES |
||
|
Probe RT-qPCR Kit |
Universal version reagent |
HifairTM V2 Multiplex One Step RT-qPCR Probe Kit (UDG Plus, GC enhancer plus) |
16630ES |
|
"one-tube" fully premixed reagents |
HifairTM Superpro Ⅱ One Step RT-qPCR Master Mix |
11899ES |
|
|
Lyo-Ready RT-qPCR reagent |
HifairTM Lyo-Ready I Multiplex One Step RT-qPCR Kit（UDG plus) |
16645ES |
|