1、Core enzyme material-Taq DNA Polymerase

As the expansion of application testing fields and the increase in market demand, wild-type Taq DNA polymerase can no longer fully meet the requirements. Yeasen's ZymeEditor™ enzyme modification platform has comprehensively and directionally modified Taq DNA polymerase to meet various application needs, launching a series of hot-start Taq DNA polymerases suitable for pre-mixed, inhibitor-resistant, ultra-clean, and other applications.

• Superior Stability: Formulated into a complete premix solution containing primers and probes, after treatment at 37°C for 7 days, there is no change in low-concentration detection.

Figure 1: Using HBV nucleic acid as a template, a qPCR complete premix solution was prepared with 10726ES, and all components except the template were tested for accelerated stability at 37°C for 7 days. The results showed that there were no significant changes in Ct values and fluorescence values at high, medium, and low concentrations, indicating that the premix solution prepared with 10726ES has stable performance.

• Low host gDNA residue: Residual E. coli genomic DNA is less than 0.5 copies per 100 U.

Figure 2: Linear range of E. coli genomic DNA: 30 fg/μL to 300 pg/μL, with a correlation coefficient R2 of 0.9999. (B) Residual E. coli genomic DNA in three batches of Hieff UCF.ME® Sensitive Taq DNA Polymerase is less than 0.5 copies per 100 U.

2、Core enzyme material-Reverse Transcriptase

As one of the key components of the RT-qPCR reaction, reverse transcriptase must possess faster reverse transcription speed, higher synthesis yield, and better heat resistance. To obtain reverse transcriptase more suitable for RT-qPCR, Yeasen Biotech has developed the Hifair® series of reverse transcriptases based on M-MLV through genetic modification. These enzymes feature lower RNaseH activity, higher thermal stability, and stronger continuous synthesis capabilities.

• It can withstand the reaction temperature of 58℃ and is suitable for reverse transcription of complex RNA templates

Figure 3: Hifair® V Reverse Transcriptase and the imported brand T* reverse transcriptase were incubated at 55°C and 58°C for 30 minutes to detect residual enzyme activity. The results show that Hifair® V Reverse Transcriptase maintains 70% activity after incubation at 58°C for 30 minutes, significantly exceeding the imported brand.

• Superior detection rate in RT-qPCR compared to other brands, with a detection range of 1 pg to 1 μg

Figure 4: Using 293T cell total RNA at concentrations ranging from 1 pg to 1 μg (7 gradients) as a template, reverse transcription was performed using Hifair® V one-step RT-gDNA digestion SuperMix for qPCR (Cat#11142), V*, and T*. The resulting cDNA was then subjected to qPCR for quantitative analysis. The results indicate that the sensitivity of 11142ES can reach 1 pg, with a detection rate superior to that of competing products.

3、Other Enzymes and Enzyme Inhibitors: RNase Inhibitor, UDG Enzyme

Yeasen Biotech offers a variety of enzymes and enzyme inhibitors that have been validated for IVD industrial applications, including RNase inhibitors and UDG enzymes, providing a rich selection of high-quality raw material solutions for the development and production of a wide range of in vitro diagnostic products. These enzyme-related products are widely used in the protection of molecular detection target products.

RNase inhibitor：Yeasen expresses and purifies recombinant mouse/porcine RNase inhibitors in a soluble form within E. coli, which can broadly inhibit various types of RNases (RNase A, B, C) without suppressing the activity of polymerases. These inhibitors have been validated for widespread use in multiple applications such as RT-PCR/qPCR, RNA-seq, cDNA cloning, and library construction. In addition to conventional RNase inhibitors, Yeasen has also developed RNase inhibitors processed with the UCF.ME® ultra-low residue technology, which have lower host residue and are suitable for applications with stricter requirements for background bacteria, aiding in the resolution of background bacterial interference in pathogen detection and enhancing the accuracy of detection.

Uracil DNA Glycosylase（UDG/UNG enzyme）：Uracil-DNA glycosylase (UDG enzyme), when used in conjunction with dUTP, can establish a PCR contamination prevention system. Yisheng's thermolabile, low UDG enzyme can avoid the residual activity of conventional UDG enzymes after inactivation, which may cause degradation of dU-containing amplification products at room temperature. It also prevents false positives in PCR results caused by background bacteria present in molecular enzymes. This is crucial for accurately identifying infectious pathogens and assisting in the clinical diagnosis of infections and infectious diseases.

Taq DNA Polymerase Antibody：Nonspecific amplification is one of the major issues significantly affecting the performance of PCR, and hot start technology can effectively reduce nonspecific amplification. The hot start enzyme modified with antibodies has a high sealing efficiency and excellent performance, being stable at room temperature with a fast release rate of enzyme activity. The dual-antibody hot start Taq enzyme developed by Yisheng Biotech not only seals the 5'→3' polymerase activity of Taq enzyme but also simultaneously seals the 5'→3' exonuclease activity. This dual approach effectively prevents nonspecific amplification caused by mispairing or primer dimers and also prevents the generation of nonspecific signals due to the degradation of probes or primers, thereby doubly enhancing the stability of the reagents.

4、Universal High-Performance One tube qPCR Master Mix

Yeasen Biotech offers high-quality qPCR reagents, featuring high-performance hot-start Taq polymerase paired with an optimized buffer system. The company provides a range of products, including universal master mixes, all-in-one master mixes, genotyping master mixes, and freeze-dried master mixes. These reagents are characterized by their ease of use, high sensitivity, and strong stability, which accelerate the development of your molecular diagnostic products.

• good versatility：It is suitable for multiple types of multiple primers and probes(more than 40 different types of targets have been verified), and has excellent amplification performance;
• l Super full premix stability: stabel at 37℃ for 14 days, 4℃ for 28 days, repeated freezing and thawing 50 times；

• High sensitivity and good specificity: the sensitivity can detect 0.25 copy/μL, and the specificity of 48 holes has no negative peak；
• Support fast program:compatible with fast program, 30min can produce results;
• dUP/UDG anti-pollution system: dUTP/UDG anti-pollution system is introduced to effectively prevent aerosol pollution;
• Multi-platform and multi-system application: Bio-Rad CFX96, ABI Q5, 7500, Slan, Tianlong, etc.

Figure 5: Based on the stability study of the all-in-one pre-mixed reagent, 16710ES was treated in three different ways (4°C, 37°C, and freeze-thaw cycles). The experimental groups were treated at 4°C and 37°C for 7 days, respectively, and subjected to 50 freeze-thaw cycles, while the control group (stored at -20°C) was evaluated for stability under four multiplex amplification systems (Groups 1, 2, and 4 are quadruplex amplification systems, and Group 3 is a triplex amplification system). The results demonstrated that the deviation of Ct values (ΔCt) between the experimental and control groups was within ±0.5, and the fluorescence value deviation was within 15%, proving that Yeasen's all-in-one pre-mixed reagent possesses excellent stability in multiplex amplification.

5、Universal High-Performance RT-qPCR Master Mix

One-step RT-qPCR reagents integrate the superior performance of reverse transcriptase and hot-start enzymes, complemented by an optimized buffering system, enabling rapid and sensitive detection of single-digit copy templates. Validated by various customer markets including respiratory disease, animal epidemic, and research systems, these reagents can meet the needs of various application directions.

• good versatility：It is suitable for qPCR amplification and detection of nucleic acids of pathogenic microorganisms, human genomes, plants and other species；
• High sensitivity：The carefully optimized buffer system improves the detection sensitivity of low-concentration templates up to 250 copies/mL；
• dUP/UDG anti-pollution system：dUTP/ Heat-labile UDG anti-pollution system is introduced to degrade aerosol pollution of products efficiently, reduce false positive, and ensure authentic results；
• Support fast program: compatible with fast program, 40min can produce results;
• Super stability: stabel at 37℃ for 14 days,repeated freezing and thawing 10 times.

Detection sensitivity evaluation

Figure 6: Utilizing Yeasen's RT-qPCR premix (16630ES) and a comparable product from Supplier-A, the detection of four pathogen targets was conducted simultaneously (Human Parainfluenza Virus HPIV2, Human Parainfluenza Virus HPIV4, Respiratory Syncytial Virus HRVA, and Rhinovirus RSVA). The results indicated that the detection sensitivity of 16630ES was higher than that of Supplier-A.

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